Comparison of the structural and kinetic properties of the cytochrome c nitrite reductases from Escherichia coli, Wolinella succinogenes, Sulfurospirillum deleyianum and Desulfovibrio desulfuricans

Clarke, TA ORCID: https://orcid.org/0000-0002-6234-1914, Hemmings, AM ORCID: https://orcid.org/0000-0003-3053-3134, Burlat, B, Butt, JN ORCID: https://orcid.org/0000-0002-9624-5226, Cole, JA and Richardson, DJ ORCID: https://orcid.org/0000-0002-6847-1832 (2006) Comparison of the structural and kinetic properties of the cytochrome c nitrite reductases from Escherichia coli, Wolinella succinogenes, Sulfurospirillum deleyianum and Desulfovibrio desulfuricans. Biochemical Society Transactions, 34 (1). pp. 143-145. ISSN 1470-8752

Full text not available from this repository. (Request a copy)

Abstract

The recent crystallographic characterization of NrfAs from Sulfurospirillum deleyianum, Wolinella succinogenes, Escherichia coli and Desulfovibrio desulfuricans allows structurally conserved regions to be identified. Comparison of nitrite and sulphite reductase activities from different bacteria shows that the relative activities vary according to organism. By comparison of both amino acid sequences and structures, differences can be identified in the monomer–monomer interface and the active-site channel; these differences could be responsible for the observed variance in substrate activity and indicate that subtle changes in the NrfA structure may optimize the enzyme for different roles.

Item Type: Article
Faculty \ School: Faculty of Science > School of Biological Sciences
UEA Research Groups: Faculty of Science > Research Groups > Biophysical Chemistry (former - to 2017)
Faculty of Science > Research Groups > Molecular Microbiology
Faculty of Science > Research Groups > Organisms and the Environment
Faculty of Science > Research Groups > Plant Sciences
Faculty of Science > Research Groups > Chemistry of Light and Energy
Faculty of Science > Research Groups > Chemistry of Life Processes
Faculty of Science > Research Centres > Centre for Molecular and Structural Biochemistry
Faculty of Science > Research Groups > Energy Materials Laboratory
Depositing User: EPrints Services
Date Deposited: 01 Oct 2010 13:37
Last Modified: 23 Jul 2024 00:23
URI: https://ueaeprints.uea.ac.uk/id/eprint/1162
DOI: 10.1042/BST0340143

Actions (login required)

View Item View Item