Expression of avian prickle genes during early development and organogenesis

Cooper, Oliver, Sweetman, Dylan, Wagstaff, Laura and Munsterberg, Andrea ORCID: https://orcid.org/0000-0002-4577-4240 (2008) Expression of avian prickle genes during early development and organogenesis. Developmental Dynamics, 237 (5). pp. 1442-1448. ISSN 1097-0177

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Abstract

Chicken homologues of prickle-1 (pk-1) and prickle-2 (pk-2) were isolated to gain insight into the extent of planar cell polarity signaling during avian embryogenesis. Bioinformatics analyses demonstrated homology and showed that pk-1 and pk-2 exhibited conserved synteny with ADAMTS20 and ADAMTS9, GON-related zinc metalloproteases. Expression of pk-1 and pk-2 was established during embryogenesis and early organogenesis, using in situ hybridization and sections of chicken embryos. At early stages, pk-1 was expressed in Hensen's node, primitive streak, ventral neural tube, and foregut. In older embryos, pk-1 transcripts were detected in dorsolateral epithelial somites, dorsomedial lip of dermomyotomes, and differentiating myotomes. Furthermore, pk-1 expression was seen in lateral body folds, limb buds, and ventral metencephalon. pk-2 was expressed in Hensen's node and neural ectoderm at early stages. In older embryos, pk-2 expression was restricted to ventromedial epithelial somites, except in the most recently formed somite pair, and limb bud mesenchyme.

Item Type: Article
Faculty \ School: Faculty of Science > School of Biological Sciences
Depositing User: EPrints Services
Date Deposited: 01 Oct 2010 13:36
Last Modified: 24 Oct 2022 00:41
URI: https://ueaeprints.uea.ac.uk/id/eprint/116
DOI: 10.1002/dvdy.21490

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