Phylogenetic diversity of the dddp gene for dimethylsulfoniopropionate-dependent dimethyl sulfide synthesis in mangrove soils

Peng, Mengjun, Xie, Qingyi, Hu, Huo, Hong, Kui, Todd, Jonathan D., Johnston, Andrew W.B. and Li, Youguo (2012) Phylogenetic diversity of the dddp gene for dimethylsulfoniopropionate-dependent dimethyl sulfide synthesis in mangrove soils. Canadian Journal of Microbiology, 58 (4). pp. 523-530. ISSN 0008-4166

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Abstract

The dddP gene encodes an enzyme that cleaves dimethylsulfoniopropionate (DMSP) into dimethyl sulfide (DMS) plus acrylate and has been identified in various marine bacteria and some fungi. The diversity of dddP genes was investigated by culture-independent PCR-based analysis of metagenomic DNA extracted from 4 mangrove soils in Southern China. A phylogenetic tree of 144 cloned dddP sequences comprised 7 groups, 3 of which also included dddP genes from previously identified Ddd + (DMSP-dependent DMS production) bacteria. However, most (69%) of the DddP sequences from the mangroves were in 4 other subgroups that did not include sequences from known bacteria, demonstrating a high level of diversity of this gene in these environments. Each clade contained clones from all of the sample sites, suggesting that different dddP types are widespread in mangroves of different geographical locations. Furthermore, it was found the dddP genotype distribution was remarkably influenced by the soil properties pH, available sulfur, salt, and total nitrogen.

Item Type:	Article
Uncontrolled Keywords:	clone library,dddp,diversity,mangrove soil,pcr,microbiology,immunology,applied microbiology and biotechnology,molecular biology,genetics,sdg 14 - life below water ,/dk/atira/pure/subjectarea/asjc/2400/2404
Faculty \ School:	Faculty of Science > School of Biological Sciences
Related URLs:	https://www.scopus.com/pages/publication...
Depositing User:	LivePure Connector
Date Deposited:	25 Aug 2026 13:42
Last Modified:	30 Aug 2026 23:03
URI:	https://ueaeprints.uea.ac.uk/id/eprint/104338
DOI:	10.1139/W2012-019

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