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Jin, Shen, Cenier, Aurelie, Wetzel, Daniela, Arefaine, Bethlehem, Moreno-Gonzalez, Mar, Stamouli, Marilena, Mohamad, Merianne, Lupatsii, Mariia, Ríos, Emilio, Lee, Sunjae, Zamalloa, Ane, Chokshi, Shilpa, Mardinoglu, Adil, Shoaie, Saeed, Beraza, Naiara 
ORCID: https://orcid.org/0000-0003-0718-0940, Patel, Vishal C. and Schirmer, Melanie
(2025)
Microbial collagenase activity is linked to oral–gut translocation in advanced chronic liver disease.
Nature Microbiology, 11 (1).
pp. 211-227.
ISSN 2058-5276
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Abstract
Microbiome perturbations are associated with advanced chronic liver disease (ACLD), but how microorganisms contribute to disease mechanisms is unclear. Here we analysed metagenomes of paired saliva and faecal samples from an ACLD cohort of 86 individuals, plus 2 control groups of 52 healthy individuals and 14 patients with sepsis. We identified highly similar oral and gut bacterial strains, including Veillonella and Streptococcus spp., which increased in absolute abundance in the gut of patients with ACLD compared with controls. These microbial translocators uniquely share a prtC gene encoding a collagenase-like proteinase, and its faecal abundance was a robust ACLD biomarker (area under precision-recall curve = 0.91). A mouse model of hepatic fibrosis inoculated with Veillonella and Streptococcus prtC-encoding patient isolates showed exacerbation of gut barrier impairment and hepatic fibrosis. Furthermore, faecal collagenase activity was increased in patients with ACLD and experimentally confirmed for the prtC gene of translocating Veillonella parvula. These findings establish mechanistic links between oral–gut translocation and ACLD pathobiology.
| Item Type: | Article |
|---|---|
| Additional Information: | Extended data is available for this paper at https://doi.org/10.1038/s41564-025-02223-0. |
| Faculty \ School: | Faculty of Science > School of Biological Sciences Faculty of Medicine and Health Sciences > Norwich Medical School |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Centres > Norwich Institute for Healthy Aging |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 17 Aug 2026 13:31 |
| Last Modified: | 17 Aug 2026 20:35 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/104178 |
| DOI: |
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