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Millar, Molly E., Abele, Miriam, Harris, Hannah C., Koev, Todor T. 
ORCID: https://orcid.org/0000-0002-8218-9753, Telatin, Andrea, Kiu, Raymond, Van Sinderen, Douwe, Khimyak, Yaroslav Z. ORCID: https://orcid.org/0000-0003-0424-4128, Ludwig, Christina, Hall, Lindsay J. and Warren, Frederick J.
(2026)
Bifidobacterial genes upregulated by resistant starch investigated using multi-omics have orthologs in infant gut isolates.
ISME Communications, 6 (1).
ISSN 2730-6151
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Abstract
Bifidobacterium species and strains are key members of the human gut microbiota, appearing soon after birth and persisting into adulthood. Resistant starch is an important dietary substrate for adult-associated bifidobacteria, where its fermentation supports host health. However, less is known about how different starch structures interact with bifidobacteria. Here we show that growth kinetics and gene expression differ depending on starch structure. Using detailed growth assays, genomics, and metabolomic analyses, bifidobacterial starch hydrolysis capabilities were closely associated with their CAZyme profiles. In one isolate of Bifidobacterium globosum, we identified a gene cluster encoding three multi-functional amylase enzymes complemented by several starch-binding modules, the genes and proteins of which were significantly upregulated in response to starch. Homologs of genes in the cluster were found in the genomes of bifidobacterial isolates from weaning infants providing insights into their role in the maturation process of the microbiota. Uncovering mechanisms of metabolic interaction between starch structures and bifidobacteria underscores the importance of this ecological function and potential health implications.
| Item Type: | Article |
|---|---|
| Additional Information: | Data availability: The RNA-seq raw read data can be found in the National Centre for Biotechnology Information (NCBI) database under BioProject ID: PRJNA1156008. The mass spectrometric raw files and the MaxQuant output files have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository [ 62] and can be accessed using the identifier PXD056548 (https://proteomecentral.proteomexchange.org/cgi/GetDataset?ID=PXD056548). The genomic data underlying this article are available in the NCBI repository. A full list of each bacterial strain and its accession number can be found in Supplementary Table S1. The remaining data underlying this article are available in the article and within its online supplementary material. |
| Uncontrolled Keywords: | bifidobacteria,cazymes,microbiome,resistant starch,microbiology,sdg 3 - good health and well-being ,/dk/atira/pure/subjectarea/asjc/2400/2404 |
| Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School Faculty of Science > School of Chemistry, Pharmacy and Pharmacology |
| UEA Research Groups: | Faculty of Science > Research Groups > Physical Chemistry and Pharmaceutics |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 14 Aug 2026 14:15 |
| Last Modified: | 18 Aug 2026 07:51 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/104146 |
| DOI: | 10.1093/ismeco/ycag136 |
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