The need for standardization and improved open (meta)data practices in metaproteomics

Van Den Bossche, Tim, Wolf, Maximilian, Armengaud, Jean, Arntzen, Magnus, Benndorf, Dirk, Figeys, Daniel ORCID: https://orcid.org/0000-0002-5373-7546, Grenga, Lucia, Hettich, Robert L., John, Nithu Sara, Jagtap, Pratik, Jehmlich, Nico, Kleiner, Manuel, Kunath, Benoit J., Li, Leyuan, Lipton, Mary, Mesuere, Bart, Neely, Benjamin A., Ning, Zhibin, Pedersen, Ane Laura, Perez-Riverol, Yasset, Rajan, Jeena, Schallert, Kay, Seifert, Jana, Uzzau, Sergio, Verschaffelt, Pieter, Wilmes, Paul, Vizcaíno, Juan Antonio, Martens, Lennart and Heyer, Robert (2026) The need for standardization and improved open (meta)data practices in metaproteomics. Microbiome, 14 (1). ISSN 2049-2618

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Abstract

Metaproteomics enables functional insight into microbial communities by identifying and quantifying proteins in complex samples. Yet, heterogeneous analytical workflows and the lack of standardization across experimental and bioinformatics stages hinder reproducibility and comparability, limiting integration with other omics data. We here present a community-developed reporting checklist tailored to the specific needs of metaproteomics. We also outline current efforts to enable structured and interoperable metadata capture, drawing on standards from proteomics and microbiome research wherever possible. By promoting transparent reporting and advancing metadata practices, our recommendations aim to align metaproteomics more closely with FAIR principles and support reproducible and interoperable research practices.

Item Type: Article
Additional Information: Publisher Copyright: © The Author(s) 2026.
Uncontrolled Keywords: microbiology,microbiology (medical) ,/dk/atira/pure/subjectarea/asjc/2400/2404
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups: Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health
Related URLs:
Depositing User: LivePure Connector
Date Deposited: 07 Aug 2026 08:36
Last Modified: 09 Aug 2026 05:38
URI: https://ueaeprints.uea.ac.uk/id/eprint/104031
DOI: 10.1186/s40168-026-02455-0

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