Tools
Tools
Caffrey, Brian J. 
ORCID: https://orcid.org/0000-0001-5040-4247, Pedrazo-Tardajos, Adrián, Liberti, Emanuela, Gaunt, Benjamin, Kim, Judy S. and Kirkland, Angus I.
(2024)
Liquid Phase Electron Microscopy of Bacterial Ultrastructure.
Small, 20 (50).
ISSN 1613-6810
Preview |
PDF (Small - 2024 - Caffrey - Liquid Phase Electron Microscopy of Bacterial Ultrastructure)
- Published Version
Available under License Creative Commons Attribution. Download (9MB) | Preview |
Abstract
Recent advances in liquid phase scanning transmission electron microscopy (LP-STEM) have enabled the study of dynamic biological processes at nanometer resolutions, paving the way for live-cell imaging using electron microscopy. However, this technique is often hampered by the inherent thickness of whole cell samples and damage from electron beam irradiation. These restrictions degrade image quality and resolution, impeding biological interpretation. Using graphene encapsulation, scanning transmission electron microscopy (STEM), and energy-dispersive X-ray (EDX) spectroscopy to mitigate these issues provides unprecedented levels of intracellular detail in aqueous specimens. This study demonstrates the potential of LP-STEM to examine and identify internal cellular structures in thick biological samples. Specifically, it highlights the use of LP-STEM to investigate the radiation resistant, gram-positive bacterium, Deinococcus radiodurans using various imaging techniques.
| Item Type: | Article |
|---|---|
| Additional Information: | Data Availability Statement: The data that support the findings of this study are openly available in Zenodo at 10.5281/zenodo.10625794 and 10.5281/zenodo.11527417.. |
| Uncontrolled Keywords: | deinococcus.radiodurans,energy dispersive x-ray spectroscopy,graphene encapsulation,liquid phase,manganese uptake,radiation resistance,scanning transmission electron microscopy,biotechnology,general chemistry,biomaterials,general materials science,engineering (miscellaneous) ,/dk/atira/pure/subjectarea/asjc/1300/1305 |
| Faculty \ School: | Faculty of Science > School of Biological Sciences |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 21 Jul 2026 13:08 |
| Last Modified: | 21 Jul 2026 13:08 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/103934 |
| DOI: | 10.1002/smll.202402871 |
Downloads
Downloads per month over past year
Actions (login required)
 |
View Item |