Imaging Cellular Architecture in Three Dimensions Through Electron Microscopy

Caffrey, Brian ORCID: https://orcid.org/0000-0001-5040-4247 and Subramaniam, Sriram (2022) Imaging Cellular Architecture in Three Dimensions Through Electron Microscopy. In: Encyclopedia of Cell Biology. Elsevier, pp. 51-60. ISBN 9780128216248

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Abstract

Unraveling the complex three-dimensional architecture within cells and tissues is fundamental to understanding the initial alterations which occur within disease processes. Localisation of the spatial arrangement of these organelles and their interactions at high resolutions can therefore provide information enabling early diagnosis and in turn, potentially lead to therapeutic breakthroughs. Transmission electron microscopy is a powerful tool to obtain ultrastructural detail at near-atomic resolution. For intermediate resolutions, there is great potential to use focused ion beams in combination with a scanning electron microscope to image cells and tissue at resolutions that are higher than what is possible with optical imaging methods. Here we provide an in-depth look at some of these methods and their practical application to problems in cell biology.

Item Type:	Book Section
Additional Information:	Publisher Copyright: © 2023 Elsevier Inc. All rights reserved.
Uncontrolled Keywords:	3d electron microscopy,correlative light electron microscopy,focused ion beam-scanning electron microscopy,serial block face-sem,serial-section transmission electron microscopy,general biochemistry,genetics and molecular biology ,/dk/atira/pure/subjectarea/asjc/1300/1300
Faculty \ School:	Faculty of Science > School of Biological Sciences
Related URLs:	https://www.scopus.com/pages/publication...
Depositing User:	LivePure Connector
Date Deposited:	06 Jul 2026 15:19
Last Modified:	06 Jul 2026 15:20
URI:	https://ueaeprints.uea.ac.uk/id/eprint/103677
DOI:	10.1016/B978-0-12-821618-7.00113-9

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