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van Wonderen, Jessica H., Kent, Daisy L., Emmerson, Mary E. G., Piper, Samuel E. H., Zhang, Huijie, Jeuken, Lars J. C., Sazanovich, Igor V., Towrie, Michael, Meech, Stephen R. 
ORCID: https://orcid.org/0000-0001-5561-2782 and Butt, Julea N. ORCID: https://orcid.org/0000-0002-9624-5226
(2026)
Engineering Cytochromes for Photocatalysis: Biohybrid Assemblies for Light-Driven Dye Decoloration.
New Biotechnology.
ISSN 1871-6784
(In Press)
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Abstract
A major challenge in developing effective photocatalysts lies in engineering the efficient coupling of one-electron photochemistry with the multi-electron requirements of chemical transformations. Here we demonstrate biohybrid assemblies that achieve this key performance requirement by storing photoenergized electrons on multiple heme cofactors within the MtrC enzyme which catalyzes azo dye reduction. The biohybrid assemblies were created by site-selective labeling of MtrC with a Ru(II) (bipyridine)3 photosensitizer dye. Photocatalytic azo dye reduction and decoloration occurred when these assemblies were irradiated in the presence of a sacrificial electron donor. Our Ru(II) (bipyridine)3-MtrC biohybrid assemblies operate in a manner analogous to Ru(II) (bipyridine)3-sensitized TiO2 in the sense that photoenergized electrons accumulate in the MtrC heme chain rather than in the TiO2 conduction band prior to driving reductive chemical transformations. We anticipate that decoration of the photosensitized MtrC protein with electrocatalysts (natural or synthetic) will enable the Ru(II) (bipyridine)3-MtrC assemblies to drive a wide range of light-driven reductive transformations. Thus, MtrC provides a natural alternative to TiO2 materials for which the production and disposal present significant environmental and energy impacts.
| Item Type: | Article |
|---|---|
| Additional Information: | Data Availability: Datasets used for figures are deposited at Figshare (DOI: 10.6084/m9.figshare.31362412) |
| Uncontrolled Keywords: | photocatalysis,cytochrome,bleaching,biohybrid,ru(ii) (bipyridine)3,spectroscopy,azo dye,sdg 6 - clean water and sanitation ,/dk/atira/pure/sustainabledevelopmentgoals/clean_water_and_sanitation |
| Faculty \ School: | Faculty of Science > School of Biological Sciences Faculty of Science > School of Chemistry, Pharmacy and Pharmacology |
| UEA Research Groups: | Faculty of Science > Research Groups > Wolfson Centre for Advanced Environmental Microbiology Faculty of Science > Research Groups > Molecular Microbiology Faculty of Science > Research Centres > Centre for Molecular and Structural Biochemistry |
| Depositing User: | LivePure Connector |
| Date Deposited: | 01 Jul 2026 15:30 |
| Last Modified: | 01 Jul 2026 23:02 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/103587 |
| DOI: | issn:1871-6784 |
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