Compromised lymphocytes infiltrate hepatocellular carcinoma:The role of T-regulatory cells

Unitt, Esther, Rushbrook, Simon M., Marshall, Aileen, Davies, Susan, Gibbs, Paul, Morris, Lesley S., Coleman, Nicholas and Alexander, Graeme J.M. (2005) Compromised lymphocytes infiltrate hepatocellular carcinoma:The role of T-regulatory cells. Hepatology, 41 (4). pp. 722-730. ISSN 0270-9139

Full text not available from this repository. (Request a copy)

Abstract

Hepatocellular carcinoma (HCC) has a poor prognosis with limited therapeutic options. We propose that local immune responses in patients with HCC are held in check by tumor-infiltrating CD4+CD25+ T-regulatory lymphocytes (Treg cells), which suppress the activity and proliferation of effector CD4+ and CD8+ T cells. The phenotype and cell cycle status of tumor-infiltrating lymphocytes (TILs) in HCC were analyzed via immunohistochemistry of sections from patients undergoing surgery for HCC and via flow cytometry of peripheral blood mononuclear cells and TILs isolated from patients with HCC. Circulating and tumor-infiltrating T-cell function and activation status were assessed via proliferation and flow cytometry. More than 96% of TILs were quiescent as measured via Mcm-2 or Ki-67 expression, while less than 10% of CD8+ T cells expressed perform or granzyme B. CD4+CD25+ Treg cells comprised 8.7% (1.4-13.8) of TILs and always exceeded the proportion in distant nontumor tissue (2.4% [1.5-5.6]; P = .014). Treg cells isolated from HCC suppressed proliferation of autologous circulating CD4+CD25 - cells and perforin expression and proliferation of autologous CD8+ T cells. The proportion of circulating Treg cells in patients with HCC was similar in healthy controls (7.2% [1.2-23.3] and 9.2% [1.6-30.2], respectively), but the proportion of circulating Treg cells that were also transforming growth factor β1+ was elevated in HCC compared with controls (55.5% [8.2-73.9] and 2.0% [0-4.9], respectively; P = .003). In conclusion, TILs are compromised and contain a subpopulation of suppressive CD4+CD25+Foxp3+ Treg cells. Functional deletion of tumor-infiltrating Treg cells could enhance tumor-specific immunotherapy.

Item Type:	Article
Uncontrolled Keywords:	hepatology ,/dk/atira/pure/subjectarea/asjc/2700/2721
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
Related URLs:	https://www.scopus.com/pages/publication...
Depositing User:	LivePure Connector
Date Deposited:	30 Jun 2026 13:23
Last Modified:	01 Jul 2026 07:38
URI:	https://ueaeprints.uea.ac.uk/id/eprint/103533
DOI:	10.1002/hep.20644

Actions (login required)

View Item