A novel immunohistochemical method for estimating cell cycle phase distribution in ovarian serous neoplasms:Implications for the histopathological assessment of paraffin-embedded specimens

Scott, I. S., Heath, T. M., Morris, L. S., Rushbrook, S. M., Bird, K., Vowler, S. L., Arends, M. J. and Coleman, N. (2004) A novel immunohistochemical method for estimating cell cycle phase distribution in ovarian serous neoplasms:Implications for the histopathological assessment of paraffin-embedded specimens. British Journal of Cancer, 90 (8). pp. 1583-1590. ISSN 0007-0920

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Abstract

We have investigated whether immunohistochemical markers can identify differences in cell cycle phase distribution in ovarian serous neoplasms, including borderline tumours of different grades. Sections of normal ovary (n = 18), serous cystadenoma (n = 21), borderline serous tumours (n = 21) and serous cystadenocarcinoma (n = 15) were analysed by immunohistochemistry using markers of cell cycle entry (Mcm-2) and cell cycle phase, including cyclin D1 (mid-to-late G1), cyclin A (S phase), cyclin B1 (G2 phase) and phosphohistone H3 (mitosis). Double-labelling confocal microscopy confirmed marker phase specificity and phase estimations were corroborated by flow cytometry. On progression from normal ovary through serous cystadenoma and borderline tumours to cystadenocarcinomas, expression of Mcm-2 (P < 0.0001), cyclin D1 (P = 0.002), cyclin A (P < 0.0001), cyclin B1 (P < 0.0001) and phosphohistone H3 (P < 0.0001) increased, paralleled by an increase in the S-phase fraction (cyclin A:Mcm-2 ratio; P = 0.002). Borderline tumours of increasing grade also showed increased Mcm-2 and cyclin A expression, together with an increase in the S-phase fraction. Immunohistochemistry can be used to estimate cell cycle phase distribution in ovarian serous neoplasms, giving results similar to flow cytometric analysis and enabling direct assessment of tumour heterogeneity. Immunohistochemical estimates of the S-phase fraction may identify serous borderline tumours likely to exhibit malignant progression and/or select serous cystadenocarcinomas likely to respond to adjuvant therapy.

Item Type:	Article
Uncontrolled Keywords:	borderline tumours,cell cycle,minichromosome maintenance proteins,ovary,serous cystadenocarcinoma,oncology,cancer research ,/dk/atira/pure/subjectarea/asjc/2700/2730
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
Related URLs:	https://www.scopus.com/pages/publication...
Depositing User:	LivePure Connector
Date Deposited:	30 Jun 2026 13:22
Last Modified:	30 Jun 2026 15:23
URI:	https://ueaeprints.uea.ac.uk/id/eprint/103529
DOI:	10.1038/sj.bjc.6601660

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