Profiling Antibody Reactivity to Gut Microbes in ME/CFS Patients

Seton, Katharine A. and Carding, Simon R. (2025) Profiling Antibody Reactivity to Gut Microbes in ME/CFS Patients. In: Methods in Molecular Biology. Methods in Molecular Biology . Humana Press Inc, pp. 279-293.

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Abstract

The gut microbiome plays a vital role in physiological functions including metabolism, immune regulation, and gut-brain communication. Alterations in gut microbe makeup and function, termed microbial dysbiosis, are associated with various metabolic, inflammatory, and neurological disorders. Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) patients often display gut microbial dysbiosis and increased intestinal barrier permeability (“leaky gut”). This “leaky gut" allows for microbial products and toxins, such as lipopolysaccharides (LPS), to enter the bloodstream, triggering systemic inflammation and immune dysregulation. ME/CFS patients exhibit altered immune responses, including production of antibodies reactive with gut microbial antigens, although the significance of these antibodies in promoting pathogenic or protective immune responses remains unclear. This chapter outlines methodologies for quantifying antibody reactivity to intestinal microbes and identifying stool-bound IgG in ME/CFS patients and healthy same household controls, to further investigate the role of anti-microbial IgG in ME/CFS pathogenesis.

Item Type:	Book Section
Additional Information:	Publisher Copyright: © The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2025.
Uncontrolled Keywords:	chronic fatigue syndrome,elisa,flow cytometry,iga,igg,igg-seq,microbes,microbiome,myalgic encephalomyelitis,molecular biology,genetics ,/dk/atira/pure/subjectarea/asjc/1300/1312
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
Related URLs:	https://www.scopus.com/pages/publication...
Depositing User:	LivePure Connector
Date Deposited:	03 Jun 2026 14:59
Last Modified:	06 Jun 2026 13:51
URI:	https://ueaeprints.uea.ac.uk/id/eprint/103265
DOI:	10.1007/978-1-0716-4498-0_16

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