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ToolsStentz, Régis, Jones, Emily, Gul, Lejla, Latousakis, Dimitrios, Parker, Aimee, Brion, Arlaine, Goldson, Andrew J., Gotts, Kathryn and Carding, Simon R. (2025) Proteomics of Bacterial and Mouse Extracellular Vesicles Released in the Gastrointestinal Tracts of Nutrient-Stressed Animals Reveals an Interplay Between Microbial Serine Proteases and Mammalian Serine Protease Inhibitors. International Journal of Molecular Sciences, 26 (9). pp. 1-23. ISSN 1661-6596
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Abstract
Bacterial extracellular vesicles (BEVs) produced by members of the intestinal microbiota can not only contribute to digestion but also mediate microbe–host cell communication via the transfer of functional biomolecules to mammalian host cells. An unresolved question is which host factors and conditions influence BEV cargo and how they impact host cell function. To address this question, we analysed and compared the proteomes of BEVs released by the major human gastrointestinal tract (GIT) symbiont Bacteroides thetaiotaomicron (Bt) in vivo in fed versus fasted animals using nano-liquid chromatography with tandem mass spectrometry (LC-MSMS). Among the proteins whose abundance was negatively affected by fasting, nine of ten proteins of the serine protease family, including the regulatory protein dipeptidyl peptidase-4 (DPP-4), were significantly decreased in BEVs produced in the GITs of fasted animals. Strikingly, in extracellular vesicles produced by the intestinal epithelia of the same fasted mice, the proteins with the most increased abundance were serine protease inhibitors (serpins). Together, these findings suggest a dynamic interaction between GI bacteria and the host. Additionally, they indicate a regulatory role for the host in determining the balance between bacterial serine proteases and host serpins exported in bacterial and host extracellular vesicles.
| Item Type: | Article |
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| Additional Information: | Data Availability Statement The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE [75] partner repository with the dataset identifier PXD062737. All other data used to support the findings of this study are included within the article. Acknowledgments We gratefully acknowledge the Proteomics Facility at the University of Bristol, under the leadership of Kate Heesom, for their support in performing the mass spectrometry analysis and providing expert guidance throughout this study. We also thank the John Innes Centre Bioimaging Facility and staff for their contribution to this publication. |
| Uncontrolled Keywords: | bacterial extracellular vesicles,bacteroides thetaiotaomicron,intestine,microbiota,nutrition,proteome,catalysis,molecular biology,spectroscopy,computer science applications,physical and theoretical chemistry,organic chemistry,inorganic chemistry ,/dk/atira/pure/subjectarea/asjc/1500/1503 |
| Faculty \ School: | Faculty of Science > School of Biological Sciences Faculty of Medicine and Health Sciences > Norwich Medical School |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Centres > Norwich Institute for Healthy Aging Faculty of Medicine and Health Sciences > Research Groups > Gastroenterology and Gut Biology Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health |
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| Depositing User: | LivePure Connector |
| Date Deposited: | 19 May 2026 12:42 |
| Last Modified: | 19 May 2026 12:42 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/103095 |
| DOI: | 10.3390/ijms26094080 |
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