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ToolsAssis, Dener Acosta de, Warren, Martin, Kroon, Paul and Ayub, Marco Antônio Záchia (2025) Delivery system of vitamin B12 in plant-based food products from Propionibacterium freudenreichii subsp. shermanii ATCC 13673 grown in agro-industrial by-products. Food Bioscience, 71. pp. 1-8. ISSN 2212-4292
Full text not available from this repository. (Request a copy)Abstract
Plant-based diets are emerging worldwide, potentially exposing an increasing number of people to vitamin B12 deficiency. In this study, we investigated the application of P. shermanii ATCC 13673 spray-dried biomass as a source of vitamin B12 to fortify a plant-based food, in this case, bread. Vitamin B12 characterization by LC-MS/MS system, bread fortification, and biocompatibility assays were performed. Vitamin B12 in P. shermanii ATCC 13673 spray-dried biomass samples (up to 18 μg/g) was found mainly in the hydroxycobalamin form. Bread samples fortified with P. shermanii ATCC 13673 spray-dried biomass retained two-thirds of vitamin B12 after baking. Additionally, the vitamin B12 was as bioaccessible as the cyanocobalamin commercial standard, which was added to control fortified breads. Moreover, biocompatibility assays (cytotoxicity and monolayer integrity) showed that bread samples fortified with P. shermanii ATCC 13673 spray-dried biomass did not damage Caco-2 cells, indicating its potential as a source of alternative, safe, and available vitamin B12 in plant-based foods.
| Item Type: | Article |
|---|---|
| Additional Information: | Data availability Data will be made available on request. |
| Uncontrolled Keywords: | bioprocess,cobalamin,innovation,micronutrients,vegans,food science,biochemistry ,/dk/atira/pure/subjectarea/asjc/1100/1106 |
| Faculty \ School: | Faculty of Science Faculty of Medicine and Health Sciences > Norwich Medical School Faculty of Science > School of Biological Sciences |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Centres > Norwich Institute for Healthy Aging |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 19 May 2026 10:55 |
| Last Modified: | 19 May 2026 10:55 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/103089 |
| DOI: | 10.1016/j.fbio.2025.107347 |
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