Association of antimicrobial resistant Vibrio and species pathogenic to humans with aquacultured seafood

Bloomfield, Samuel J., Palau, Raphaёlle, Janecko, Nicol, Baker-Austin, Craig and Mather, Alison E. (2025) Association of antimicrobial resistant Vibrio and species pathogenic to humans with aquacultured seafood. Food Microbiology, 132. pp. 1-12. ISSN 0740-0020

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Abstract

Vibrio is a genus of bacteria commonly found on seafood products and includes many important human pathogens. Most seafood is produced using aquaculture systems, which frequently use antimicrobial agents. Here we aimed to determine if method of seafood production was associated with Vibrio pathogenic to humans and/or antimicrobial resistant (AMR) Vibrio. Retail prawn and salmon samples that were produced using aquaculture or were wild-caught were cultured for Vibrio spp. Isolates were sequenced to identify the species and AMR genes (ARGs) present, followed by long-read sequencing of a subset of genomes to identify mobile genetic elements (MGEs). Vibrio was cultured from 136/279 of prawn and 4/157 of salmon samples, and ARG-containing Vibrio and Vibrio pathogenic to humans were associated with aquacultured prawn samples. A quarter of ARGs were found on plasmids, mostly in close vicinity to the insertion sequence type IS6/IS26. Most intrinsic chromosomal ARGs were not associated with an MGE, but most acquired chromosome ARGs were associated with a MGE, most commonly IS91. Vibrio isolates belonging to different species contained ARGs associated with similar MGEs. Vibrio has an arsenal of MGEs that can facilitate the spread of ARGs. Aquaculture practices may need to be adjusted in order to prevent the spread of AMR Vibrio and Vibrio pathogenic to humans.

Item Type:	Article
Additional Information:	We thank the Quadram Institute Bioscience core facilities sequencing and bioinformatics teams, laboratory technicians, media preparation and laboratory managers for their assistance in the project, and the CLIMB-BIG-DATA-computing servers, an infrastructure supported by the UKRI Medical Research Council grant MR/T030062/1. Raw Illumina reads were uploaded to the Sequence Read Archive (SRA) under bioprojects: PRJNA699735, PRJNA1107692 and PRJNA1155317. Raw MinION reads were uploaded to SRA under project PRJNA1155659.
Uncontrolled Keywords:	antimicrobial resistance,aquaculture,genomics,plasmids,seafood,vibrio,microbiology,food science ,/dk/atira/pure/subjectarea/asjc/2400/2404
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health
Related URLs:	https://www.scopus.com/pages/publication...
Depositing User:	LivePure Connector
Date Deposited:	05 May 2026 09:33
Last Modified:	05 May 2026 09:33
URI:	https://ueaeprints.uea.ac.uk/id/eprint/102890
DOI:	10.1016/j.fm.2025.104819

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