Activated T lymphocytes support osteoclast formation in vitro

Horwood, Nicole J. ORCID: https://orcid.org/0000-0002-6344-1677, Kartsogiannis, Vicky, Quinn, Julian M. W., Romas, Evangelos, Martin, T. John and Gillespie, Matthew T. (1999) Activated T lymphocytes support osteoclast formation in vitro. Biochemical and Biophysical Research Communications, 265 (1). pp. 144-150. ISSN 0006-291X

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Abstract

Osteoblastic stromal cells are capable of supporting osteoclast formation from hematopoietic precursors in the presence of osteotropic factors such as 1alpha,25(OH)(2)D(3), PTH, and IL-11. Osteoblastic stromal cells produce receptor activator of NF-kappaB ligand (RANKL), a type II membrane protein of the TNF ligand family, in response to these agents. Activated T lymphocytes also produce RANKL; however, the ability of this cell type to support osteoclast formation in vitro is unknown. Human PBMC-derived T cells, extracted using alphaCD3-coated magnetic beads, were cocultured with adherent murine spleen cells in the presence of Con A and a panel of cytokines. In the presence of Con A, bona fide osteoclasts were formed in vitro with activated T cells: IL-1alpha and TGFbeta further enhanced osteoclast numbers. PBMC-derived lymphocytes showed an increase in the mRNA expression of RANKL within 24 h of treatment with the same agents that were used to induce osteoclast formation. In synovial tissue sections with lymphoid infiltrates from RA patients, the expression of RANKL was demonstrated in CD3(+) T cells. The ability of activated T lymphocytes to support osteoclast formation may provide a mechanism for the potentiation of osteoclast formation and bone resorption in disease states such as rheumatoid arthritis.

Item Type:	Article
Additional Information:	Copyright 1999 Academic Press.
Uncontrolled Keywords:	aged,animals,animals, newborn,immunology,biosynthesis,cell differentiation,coculture techniques,pharmacology,female,gene expression regulation,cytology,humans,pharmacology,lymphocyte activation,male,biosynthesis,mice,mice, inbred c57bl,middle aged,cytology,rank ligand,genetics,receptor activator of nuclear factor-kappa b,cytology,immunology,immunology,drug effects,pharmacology,sdg 3 - good health and well-being ,/dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health
Depositing User:	LivePure Connector
Date Deposited:	06 Mar 2019 09:30
Last Modified:	19 Oct 2023 02:22
URI:	https://ueaeprints.uea.ac.uk/id/eprint/70117
DOI:	10.1006/bbrc.1999.1623

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