Whole-cell circular dichroism difference spectroscopy reveals an in vivo-specific deca-heme conformation in bacterial surface cytochromes

Tokunou, Yoshihide, Chinotaikul, Punthira, Hattori, Shingo, Clarke, Thomas A., Shi, Liang, Hashimoto, Kazuhito, Ishii, Kazuyuki and Okamoto, Akihiro (2018) Whole-cell circular dichroism difference spectroscopy reveals an in vivo-specific deca-heme conformation in bacterial surface cytochromes. Chemical Communications, 54. pp. 13933-13936. ISSN 1359-7345

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    Abstract

    We established whole-cell circular dichroism difference spectroscopy to identify the inter-heme interaction in deca-heme cytochrome protein MtrC in whole cell. Our data showed that the heme alignment of reduced MtrC in whole cell is distinct from that in purified one, suggesting the in vivo specific electron transport kinetics.

    Item Type: Article
    Faculty \ School: Faculty of Science > School of Biological Sciences
    Faculty of Science > School of Chemistry
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    Depositing User: LivePure Connector
    Date Deposited: 06 Dec 2018 16:30
    Last Modified: 12 Dec 2018 11:30
    URI: https://ueaeprints.uea.ac.uk/id/eprint/69188
    DOI: 10.1039/C8CC06309E

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