Simultaneous time-varying systemic appearance of oral and hepatic glucose in adults monitored with stable isotopes

Livesey, Geoff, Wilson, Peter, Dainty, Jack R., Brown, Jackie, Faulks, Richard, Roe, Mark, Newman, Tracey, Eagles, John, Mellon, Fred and Greenwood, Richard (1998) Simultaneous time-varying systemic appearance of oral and hepatic glucose in adults monitored with stable isotopes. American Journal of Physiology - Endocrinology and Metabolism, 275 (4 Pt 1). E717-E728. ISSN 0193-1849

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Abstract

The rates (and extent) of appearance of glucose in arterialized plasma from an oral glucose load and from liver (RaO, RaH) can be estimated in humans using radioisotopes, but estimates vary among laboratories. We investigated the use of stable isotopes and undertook 22 primed intravenous infusions of D-[6,6-2H2]glucose with an oral load including D-[13C6]glucose in healthy humans. The effective glucose pool volume (VS) had a lower limit of 230 ml/kg body weight (cf. 130 ml/kg commonly assumed). This VS in Steele's one-compartment model of glucose kinetics gave a systemic appearance from a 50-g oral glucose load per 70 kg body weight of 96 +/- 3% of that ingested, which compared with a theoretical value of approximately 95%. Mari's two-compartment model gave 100 +/- 3%. The two models gave practically identical RaO and RaH at each point in time and a plateau in the cumulative RaO when absorption was complete. Less than 3% of 13C was recycled to [13C3]glucose, suggesting that recycling errors were practically negligible in this study. Causes of variation among laboratories are identified. We conclude that stable isotopes provide a reliable and safe alternative to radioactive isotopes in these studies.

Item Type: Article
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
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Depositing User: Pure Connector
Date Deposited: 04 Jun 2018 13:30
Last Modified: 25 Jul 2018 14:55
URI: https://ueaeprints.uea.ac.uk/id/eprint/67275
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