Redox-dependent control of i-Motif DNA structure using copper cations

Abdelhamid, Mahmoud AS, Fábián, László, MacDonald, Colin J, Cheesman, Myles R, Gates, Andrew J and Waller, Zoë AE (2018) Redox-dependent control of i-Motif DNA structure using copper cations. Nucleic Acids Research, 46 (12). 5886–5893. ISSN 0305-1048

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    Abstract

    Previous computational studies have shown that Cu+ can act as a substitute for H+ to support formation of cytosine (C) dimers with similar conformation to the hemi-protonated base pair found in i-motif DNA. Through a range of biophysical methods, we provide experimental evidence to support the hypothesis that Cu+ can mediate C–C base pairing in i-motif DNA and preserve i-motif structure. These effects can be reversed using a metal chelator, or exposure to ambient oxygen in the air that drives oxidation of Cu+ to Cu2+, a comparatively weak ligand. Herein, we present a dynamic and redox-sensitive system for conformational control of an i-motif forming DNA sequence in response to copper cations.

    Item Type: Article
    Faculty \ School: Faculty of Science > School of Pharmacy
    Faculty of Science
    Faculty of Science > School of Chemistry
    Faculty of Science > School of Biological Sciences
    Depositing User: Pure Connector
    Date Deposited: 29 May 2018 11:31
    Last Modified: 01 May 2019 00:52
    URI: https://ueaeprints.uea.ac.uk/id/eprint/67211
    DOI: 10.1093/nar/gky390

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