Ceftriaxone resistant Salmonella Typhi carries an IncI1-ST31 plasmid encoding CTXM-15

Djeghout, Bilal, Saha, Senjuti, Islam Sajib, Mohammad Saiful, Islam, Maksuda, Kay, Gemma L., Langridge, Gemma C., Wain, John and Saha, Samir K. (2018) Ceftriaxone resistant Salmonella Typhi carries an IncI1-ST31 plasmid encoding CTXM-15. Journal of Medical Microbiology, 67 (5). pp. 620-627. ISSN 0022-2615

[img] PDF (Accepted manuscript) - Submitted Version
Restricted to Repository staff only until 04 April 2019.

Download (1855kB) | Request a copy

    Abstract

    Purpose: Ceftriaxone is the drug of choice for typhoid fever and the emergence of resistant Salmonella Typhi raises major concerns for treatment. There are an increasing number of sporadic reports of ceftriaxone resistant S. Typhi and limiting the risk of treatment failure in the patient and outbreaks in the community must be prioritised. This study describes the use of whole genome sequencing to guide outbreak identification and case management. Methodology: An isolate of ceftriaxone resistant S. Typhi from the blood of a child taken in 2011 at the Popular Diagnostic Center, Dhaka, Bangladesh was subjected to whole genome sequencing, using an Illumina NextSeq 500 and analysis using Geneious software. Results: Comparison with other ceftriaxone resistant S. Typhi revealed an isolate from the Democratic Republic of the Congo in 2015 as the closest relative but no evidence of an outbreak. A plasmid belonging to incompatibility group I1 (IncI1-ST31) which included blaCTX-M-15 (ceftriaxone resistance) associated with ISEcp-1 was identified. High similarity (90%) was seen with pS115, an IncI1 plasmid from S. Enteritidis, and with pESBL- EA11, an incI1 plasmid from E. coli (99%) showing that S. Typhi has access to ceftriaxone resistance through the acquisition of common plasmids. Conclusions: The transmission of ceftriaxone resistance from E. coli to S. Typhi is of concern because of clinical resistance to ceftriaxone, the main stay of typhoid treatment. Whole genome sequencing, albeit several years after the isolation, demonstrated the success of containment but clinical trials with alternative agents are urgently required.

    Item Type: Article
    Uncontrolled Keywords: salmonella typhi,ceftriaxone resistance,antibiotic resistance,ctx-m-15,inci1-st31 plasmid,bangladesh
    Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
    University of East Anglia > Faculty of Medicine and Health Sciences > Research Groups > Medical Microbiology
    Depositing User: Pure Connector
    Date Deposited: 26 Mar 2018 15:30
    Last Modified: 25 Jul 2018 14:42
    URI: https://ueaeprints.uea.ac.uk/id/eprint/66602
    DOI: 10.1099/jmm.0.000727

    Actions (login required)

    View Item