Molecular Basis for Specificity of the Extracytoplasmic Thioredoxin ResA

Lewin, A., Crow, A., Oubrie, A. and Le Brun, N.E. (2006) Molecular Basis for Specificity of the Extracytoplasmic Thioredoxin ResA. Journal of Biological Chemistry, 281. pp. 35467-35477. ISSN 1083-351X

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Abstract

ResA, an extracytoplasmic thioredoxin from Bacillus subtilis, acts in cytochrome c maturation by reducing the disulfide bond present in apocytochromes prior to covalent attachment of heme. This reaction is (and has to be) specific, as broad substrate specificity would result in unproductive shortcircuiting with the general oxidizing thioredoxin(s) present in the same compartment. Using mutational analysis and subsequent biochemical and structural characterization of active site variants, we show that reduced ResA displays unusually low reactivity at neutral pH, consistent with the observed high pKa values >8 for both active site cysteines. Residue Glu80 is shown to play a key role in controlling the acid-base properties of the active site. A model in which substrate binding dramatically enhances the reactivity of the active site cysteines is proposed to account for the specificity of the protein. Such a substratemediated activation mechanism is likely to have wide relevance for extracytoplasmic thioredoxins.

Item Type: Article
Faculty \ School: Faculty of Science > School of Chemistry
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Depositing User: Pure Connector
Date Deposited: 24 Jan 2018 17:30
Last Modified: 04 Dec 2018 10:30
URI: https://ueaeprints.uea.ac.uk/id/eprint/66070
DOI: 10.1074/jbc.M607047200

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