Analyzing the Complex Regulatory Landscape of Hfq – an Integrative, Multi-Omics Approach

Grenga, Lucia, Chandra, Govind, Saalbach, Gerhard, Galmozzi, Carla V., Kramer, Günter and Malone, Jacob G. (2017) Analyzing the Complex Regulatory Landscape of Hfq – an Integrative, Multi-Omics Approach. Frontiers in Microbiology, 8. ISSN 1664-302X

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    Abstract

    The ability of bacteria to respond to environmental change is based on the ability to coordinate, redirect and fine-tune their genetic repertoire as and when required. While we can learn a great deal from reductive analysis of individual pathways and global approaches to gene regulation, a deeper understanding of these complex signaling networks requires the simultaneous consideration of several regulatory layers at the genome scale. To highlight the power of this approach we analyzed the Hfq transcriptional/translational regulatory network in the model bacterium Pseudomonas fluorescens. We first used extensive ‘omics’ analyses to assess how hfq deletion affects mRNA abundance, mRNA translation and protein abundance. The subsequent, multi-level integration of these datasets allows us to highlight the discrete contributions by Hfq to gene regulation at different levels. The integrative approach to regulatory analysis we describe here has significant potential, for both dissecting individual signaling pathways and understanding the strategies bacteria use to cope with external challenges.

    Item Type: Article
    Uncontrolled Keywords: pseudomonas,ribosomal profiling,multi-omics analysis,hfq,integrative approach
    Faculty \ School: Faculty of Science > School of Biological Sciences
    University of East Anglia > Faculty of Science > Research Groups > Molecular Microbiology
    Related URLs:
    Depositing User: Pure Connector
    Date Deposited: 30 Sep 2017 06:05
    Last Modified: 25 Jul 2018 14:07
    URI: https://ueaeprints.uea.ac.uk/id/eprint/65007
    DOI: 10.3389/fmicb.2017.01784

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