Carrion fly-derived DNA metabarcoding is an effective tool for mammal surveys: Evidence from a known tropical mammal community

Rodgers, Torrey W., Xu, Charles C. Y., Giacalone, Jacalyn, Kapheim, Karen M., Saltonstall, Kristin, Vargas, Marta, Yu, Douglas W., Somervuo, Panu, McMillan, W. Owen and Jansen, Patrick A. (2017) Carrion fly-derived DNA metabarcoding is an effective tool for mammal surveys: Evidence from a known tropical mammal community. Molecular Ecology Resources, 17 (6). e133–e145. ISSN 1755-098X

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    Abstract

    Metabarcoding of vertebrate DNA derived from carrion flies has been proposed as a promising tool for biodiversity monitoring. To evaluate its efficacy, we conducted metabarcoding surveys of carrion flies on Barro Colorado Island (BCI), Panama, which has a well-known mammal community, and compared our results against diurnal transect counts and camera trapping. We collected 1,084 flies in 29 sampling days, conducted metabarcoding with mammal-specific (16S) and vertebrate-specific (12S) primers, and sequenced amplicons on Illumina MiSeq. For taxonomic assignment, we compared blast with the new program protax, and we found that protax improved species identifications. We detected 20 mammal, four bird, and one lizard species from carrion fly metabarcoding, all but one of which are known from BCI. Fly metabarcoding detected more mammal species than concurrent transect counts (29 sampling days, 13 species) and concurrent camera trapping (84 sampling days, 17 species), and detected 67% of the number of mammal species documented by 8 years of transect counts and camera trapping combined, although fly metabarcoding missed several abundant species. This study demonstrates that carrion fly metabarcoding is a powerful tool for mammal biodiversity surveys and has the potential to detect a broader range of species than more commonly used methods.

    Item Type: Article
    Uncontrolled Keywords: barro colorado island,biodiversity,camera trapping,environmental dna,transect counts
    Faculty \ School: Faculty of Science > School of Biological Sciences
    Related URLs:
    Depositing User: Pure Connector
    Date Deposited: 02 Sep 2017 06:07
    Last Modified: 11 Jun 2019 11:33
    URI: https://ueaeprints.uea.ac.uk/id/eprint/64733
    DOI: 10.1111/1755-0998.12701

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