A role for the cell-wall protein silacidin in cell size of the diatom Thalassiosira pseudonana

Kirkham, Amy, Richthammer, Patrick, Schmidt, Katrin, Wustman, Martin, Maeda, Yoshiaki, Hedrich, Rene, Brunner, Eike, Tanaka, Tsuyoshi, van Pee, Karl-Heinz, Falciatore, Angela and Mock, Thomas (2017) A role for the cell-wall protein silacidin in cell size of the diatom Thalassiosira pseudonana. The ISME Journal, 11 (11). pp. 2452-2464. ISSN 1751-7362

[img] PDF (Accepted_manuscript) - Submitted Version
Restricted to Repository staff only until 31 December 2099.
Available under License ["licenses_description_other" not defined].

Download (1142kB) | Request a copy
    [img] PDF (ismej2017100a) - Published Version
    Available under License Creative Commons Attribution.

    Download (1427kB)

      Abstract

      Diatoms contribute 20% of global primary production and form the basis of many marine food webs. Although their species diversity correlates with broad diversity in cell size, there is also an intraspecific cell-size plasticity due to sexual reproduction and varying environmental conditions. However, despite the ecological significance of the diatom cell size for food-web structure and global biogeochemical cycles, our knowledge about genes underpinning the size of diatom cells remains elusive. Here, a combination of reverse genetics, experimental evolution and comparative RNA8 sequencing analyses enabled us to identify a previously unknown genetic control of cell size in the diatom Thalassiosira pseudonana. In particular, the targeted deregulation of the expression of the cell-wall protein silacidin caused a significant increase in valve diameter. Remarkably, the natural downregulation of the silacidin gene transcript due to experimental evolution under low temperature also correlated with cell-size increase. Our data give first evidence for a genetically controlled regulation of cell size in Thalassiosira pseudonana and possibly other centric diatoms as they also encode the silacidin gene in their genomes.

      Item Type: Article
      Faculty \ School: Faculty of Science > School of Environmental Sciences
      Depositing User: Pure Connector
      Date Deposited: 21 Jul 2017 06:03
      Last Modified: 09 Apr 2019 12:18
      URI: https://ueaeprints.uea.ac.uk/id/eprint/64178
      DOI: 10.1038/ismej.2017.100

      Actions (login required)

      View Item