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Doumith, Michel, Mushtaq, Shazad, Martin, Veronique, Chaudhry, Aiysha, Adkin, Rachael, Coehlo, Juliana, Chalker, Vicki, MacGowan, Alasdair, Woodford, Neil and Livermore, David M 
ORCID: https://orcid.org/0000-0002-9856-3703
and
BSAC Resistance Surveillance Standing Committee
(2017)
Genomic sequences of Streptococcus agalactiae with high-level gentamicin resistance, collected in the BSAC bacteraemia surveillance.
Journal of Antimicrobial Chemotherapy, 72 (10).
2704–2707.
ISSN 0305-7453
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Abstract
Background: Like other streptococci, Streptococcus agalactiae typically has intrinsic low-level aminoglycoside resistance. High-level gentamicin resistance was seen in 2 of 1125 isolates collected in the BSAC Bacteraemia Surveillance Programme between 2001 and 2014. These organisms, both isolated in 2014, were characterized. Methods: Identifications were by latex agglutination, MICs by BSAC agar dilution and sequencing by Illumina methodology. Results: Gentamicin MICs were >1024 mg/L versus a species mode of 8 mg/L; both isolates also were unusually ciprofloxacin resistant with MICs of 64 mg/L versus a species mode of 1 mg/L. They were distinct by sequence, but both belonged to the ST19 clone, which occurs globally. Both had aac(6′)-aph(2″), carried by different transposons, explaining their gentamicin resistance, and had gyrA[81:S-L];parC[79:S-Y], accounting for ciprofloxacin resistance. Conclusions: These are the first multiresistant S. agalactiae with the bifunctional AAC(6′)-APH(2″) enzyme to be reported in the UK for >10 years. Despite belonging to the same clonal complex, the two isolates and their resistance transposons were distinct. Both retained full susceptibility to penicillin, but any penicillin/gentamicin synergy is likely to be lost.
| Item Type: | Article |
|---|---|
| Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Groups > Epidemiology and Public Health Faculty of Medicine and Health Sciences > Research Groups > Public Health and Health Services Research (former - to 2023) |
| Depositing User: | Pure Connector |
| Date Deposited: | 13 Jul 2017 05:05 |
| Last Modified: | 03 Nov 2022 15:43 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/64097 |
| DOI: | 10.1093/jac/dkx207 |
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