Elevated matrix metalloproteinase-2 and -3 production from human diabetic dermal fibroblasts

Wall, S.J., Sampson, M.J., Levell, N. and Murphy, G. (2003) Elevated matrix metalloproteinase-2 and -3 production from human diabetic dermal fibroblasts. British Journal of Dermatology, 149 (1). pp. 13-16. ISSN 1365-2133

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Abstract

BACKGROUND: Diabetic foot ulcers are characterized by elevated levels of matrix metalloproteinases (MMP), which could lead to excessive matrix breakdown and disruption to healing. It is unknown if this elevation is a function of wound healing, or if it is present within normal skin and a primary contributor to the increased risk of impaired healing. OBJECTIVES: To determine whether diabetic fibroblasts from unwounded skin show elevated MMP production compared with their nondiabetic counterparts. PATIENTS AND METHODS: Circular skin biopsies (4 mm diameter) were taken from the inside upper arm of four controls without diabetes and from four subjects with insulin-treated diabetes. Fibroblasts were incubated for a further 72 h and conditioned medium was collected and stored at -20 degrees C. The conditioned medium was assessed by gelatin zymography and Western blotting for MMP-2 and MMP-3. RESULTS: Diabetic dermal fibroblasts showed significantly elevated production of MMP-2 (P < 0.05) and pro-MMP-3 (P < 0.05) when compared with their nondiabetic counterparts. CONCLUSIONS: Dermal fibroblasts from normal unwounded skin are characterized by increased MMP production and this may be a primary contributing factor to the increased risk of nonhealing foot ulceration in diabetes.

Item Type: Article
Uncontrolled Keywords: adult,cultured cells,conditioned culture media,diabetes mellitus,female,fibroblasts,humans,male,matrix metalloproteinase 2,matrix metalloproteinase 3,middle aged,skin,wound healing
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
Faculty of Science > School of Biological Sciences
Depositing User: EPrints Services
Date Deposited: 01 Oct 2010 14:37
Last Modified: 21 Mar 2019 10:55
URI: https://ueaeprints.uea.ac.uk/id/eprint/640
DOI: 10.1046/j.1365-2133.2003.05262.x

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