Methanethiol-dependent dimethylsulfide production in soil environments

Carrión, Ornella, Pratscher, Jennifer, Curson, Andrew R. J., Williams, Beth T. ORCID: https://orcid.org/0000-0002-8594-1269, Rostant, Wayne G. ORCID: https://orcid.org/0000-0002-3798-6251, Murrell, J. Colin and Todd, Jonathan D. (2017) Methanethiol-dependent dimethylsulfide production in soil environments. The ISME Journal, 11 (10). 2379–2390. ISSN 1751-7362

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Abstract

Dimethylsulfide (DMS) is an environmentally important trace gas with roles in sulfur cycling, signalling to higher organisms and in atmospheric chemistry. DMS is believed to be predominantly produced in marine environments via microbial degradation of the osmolyte dimethylsulfoniopropionate (DMSP). However, significant amounts of DMS are also generated from terrestrial environments, for example, peat bogs can emit ~6 μmol DMS m−2 per day, likely via the methylation of methanethiol (MeSH). A methyltransferase enzyme termed ‘MddA’, which catalyses the methylation of MeSH, generating DMS, in a wide range of bacteria and some cyanobacteria, may mediate this process, as the mddA gene is abundant in terrestrial metagenomes. This is the first study investigating the functionality of MeSH-dependent DMS production (Mdd) in a wide range of aerobic environments. All soils and marine sediment samples tested produced DMS when incubated with MeSH. Cultivation-dependent and cultivation-independent methods were used to assess microbial community changes in response to MeSH addition in a grassland soil where 35.9% of the bacteria were predicted to contain mddA. Bacteria of the genus Methylotenera were enriched in the presence of MeSH. Furthermore, many novel Mdd+ bacterial strains were isolated. Despite the abundance of mddA in the grassland soil, the Mdd pathway may not be a significant source of DMS in this environment as MeSH addition was required to detect DMS at only very low conversion rates.

Item Type:	Article
Uncontrolled Keywords:	sdg 14 - life below water ,/dk/atira/pure/sustainabledevelopmentgoals/life_below_water
Faculty \ School:	Faculty of Science > School of Environmental Sciences Faculty of Science > School of Biological Sciences University of East Anglia Research Groups/Centres > Theme - ClimateUEA
UEA Research Groups:	Faculty of Science > Research Groups > Environmental Biology Faculty of Science > Research Centres > Centre for Ecology, Evolution and Conservation Faculty of Science > Research Groups > Molecular Microbiology
Depositing User:	Pure Connector
Date Deposited:	22 Jun 2017 05:05
Last Modified:	07 Mar 2024 02:15
URI:	https://ueaeprints.uea.ac.uk/id/eprint/63799
DOI:	10.1038/ismej.2017.105

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