Transcriptional regulation of male-sterility in 7B-1 male-sterile tomato mutant

Omidvar, Vahid, Mohorianu, Irina, Dalmay, Tamas, Zheng, Yi, Fei, Zhangjun, Pucci, Anna, Mazzucato, Andrea, Večeřová, Vendula, Sedlářova, Michaela and Fellner, Martin (2017) Transcriptional regulation of male-sterility in 7B-1 male-sterile tomato mutant. PLoS ONE, 12 (2). ISSN 1932-6203

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    Abstract

    The 7B-1 tomato (Solanum lycopersicum L. cv Rutgers) is a male-sterile mutant with enhanced tolerance to abiotic stress, which makes it a potential candidate for hybrid seed breeding and stress engineering. To underline the molecular mechanism regulating the male-sterility in 7B-1, transcriptomic profiles of the 7B-1 male-sterile and wild type (WT) anthers were studied using mRNA sequencing (RNA-Seq). In total, 768 differentially expressed genes (DEGs) were identified, including 132 up-regulated and 636 down-regulated transcripts. Gene ontology (GO) enrichment analysis of DEGs suggested a general impact of the 7B-1 mutation on metabolic processes, such as proteolysis and carbohydrate catabolic process. Sixteen candidates with key roles in regulation of anther development were subjected to further analysis using qRT-PCR and in situ hybridization. Cytological studies showed several defects associated with anther development in the 7B-1 mutant, including unsynchronized anther maturation, dysfunctional meiosis, arrested microspores, defect in callose degradation and abnormal tapetum development. TUNEL assay showed a defect in programmed cell death (PCD) of tapetal cells in 7B-1 anthers. The present study provides insights into the transcriptome of the 7B-1 mutant. We identified several genes with altered expression level in 7B-1 (including beta-1,3 glucanase, GA2oxs, cystatin, cysteine protease, pectinesterase, TA29, and actin) that could potentially regulate anther developmental processes, such as meiosis, tapetum development, and cell-wall formation/degradation.

    Item Type: Article
    Faculty \ School: Faculty of Science > School of Biological Sciences
    Faculty of Science > School of Computing Sciences
    University of East Anglia > Faculty of Science > Research Groups > Plant Sciences
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    Depositing User: Pure Connector
    Date Deposited: 01 Mar 2017 01:46
    Last Modified: 25 Jul 2018 13:22
    URI: https://ueaeprints.uea.ac.uk/id/eprint/62800
    DOI: 10.1371/journal.pone.0170715

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