Autophosphorylation-based calcium (Ca2+) sensitivity priming and Ca2+/Calmodulin inhibition of Arabidopsis thaliana Ca2+-dependent protein kinase 28 (CPK28)

Bender, Kyle W, Blackburn, R Kevin, Monaghan, Jacqueline, Derbyshire, Paul, Menke, Frank LH, Zipfel, Cyril, Goshe, Michael B, Zielinski, Raymond E and Huber, Steven C (2017) Autophosphorylation-based calcium (Ca2+) sensitivity priming and Ca2+/Calmodulin inhibition of Arabidopsis thaliana Ca2+-dependent protein kinase 28 (CPK28). The Journal of Biological Chemistry, 292 (10). pp. 3988-4002. ISSN 0021-9258

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      Abstract

      Plant calcium (Ca2+) dependent protein kinases (CPKs) are composed of a dual specificity (Ser/Thr and Tyr) kinase domain tethered to a Calmodulin-like domain (CLD) via an autoinhibitory junction (J) and represent the primary Ca2+-dependent protein kinase activities in plant systems. While regulation of CPKs by Ca2+ has been extensively studied, the contribution of autophosphorylation in the control of CPK activity is less well understood. Furthermore, whether Calmodulin (CaM) contributes to CPK regulation, as is the case for Ca2+/CaM-dependent protein kinases (CaMKs) outside the plant lineage, remains an open question. We screened a subset of plant CPKs for CaM-binding and found that CPK28 is a high-affinity Ca2+/CaM-binding protein. Using synthetic peptides and native gel electrophoresis, we coarsely mapped the CaM-binding domain to a site within the CPK28 J domain that overlaps with the known site of intramolecular interaction between the J domain and CLD. Peptide kinase activity of fully dephosphorylated CPK28 was Ca2+-responsive and inhibited by Ca2+/CaM. Using in situ autophosphorylated protein, we expand on the known set of CPK28 autophosphorylation sites, and demonstrate that, unexpectedly, autophosphorylated CPK28 had enhanced activity at physiological concentrations of Ca2+ compared to dephosphorylated protein, suggesting that autophosphorylation functions to prime CPK28 for Ca2+-activation. Furthermore, CPK28 autophosphorylation substantially reduced sensitivity of the kinase to Ca2+/CaM inhibition. Overall, our analyses uncover new complexities in the control of CPK28 and provide mechanistic support for Ca2+ signaling specificity through Ca2+ sensor priming.

      Item Type: Article
      Uncontrolled Keywords: arabidopsis thaliana,autophosphorylation,calcium calmodulin (cam),protein-protein interaction,ca2+-dependent protein kinase
      Faculty \ School: Faculty of Science > School of Biological Sciences
      University of East Anglia > Faculty of Science > Research Groups > Plant Sciences
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      Depositing User: Pure Connector
      Date Deposited: 03 Feb 2017 03:28
      Last Modified: 25 Jul 2018 13:14
      URI: https://ueaeprints.uea.ac.uk/id/eprint/62285
      DOI: 10.1074/jbc.M116.763243

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