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ToolsAssar, Shima, Schoenmakers, Inez, Koulman, Albert, Prentice, Ann and Jones, Kerry S (2016) UPLC-MS/MS Determination of Deuterated 25-Hydroxyvitamin D (d3-25OHD3) and Other Vitamin D Metabolites for the Measurement of 25OHD Half-Life. In: Multiplex Biomarker Techniques. Methods in Molecular Biology . Springer, pp. 257-265. ISBN 978-1-4939-6729-2
Full text not available from this repository. (Request a copy)Abstract
Plasma 25-hydroxyvitamin D (25OHD) half-life (25OHDt 1/2) is a dynamic marker of vitamin D metabolism that can be used to assess vitamin D expenditure and help inform vitamin D requirements. Our group recently established an approach to determine the 25OHDt 1/2 as an alternative biomarker of 25OHD expenditure in humans. The approach uses a small oral dose of stable isotope labeled 25OHD3 [3-2H-25OHD3 (6,19,19-d3)] (d3-25OHD3) (tracer), which is distinguishable from endogenous 25OHD by liquid chromatography tandem-mass spectrometry (LC-MS/MS). We report here the method, which relies on protein precipitation, purification with solid phase extraction, derivatization with 4-phenyl-1,2,4-triazoline-3,5-dione (PTAD), and determination of the compounds by isotope-dilution UPLC-MS/MS. The method proved to be rapid and sensitive (LOQ 0.2 nmol/L) for the quantification of this tracer as well as the other vitamin D metabolites: 25OHD3, 25OHD2, and 24,25(OH)2D3 in human plasma.
| Item Type: | Book Section |
|---|---|
| Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Groups > Musculoskeletal Medicine Faculty of Medicine and Health Sciences > Research Groups > Nutrition and Preventive Medicine |
| Depositing User: | Pure Connector |
| Date Deposited: | 21 Dec 2016 00:04 |
| Last Modified: | 21 Oct 2022 23:59 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/61840 |
| DOI: | 10.1007/978-1-4939-6730-8_22 |
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