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Jackson, Kim G., Lockyer, Stacey, Carvalho-Wells, Andrew L., Williams, Christine M., Minihane, Anne M. 
ORCID: https://orcid.org/0000-0001-9042-4226 and Lovegrove, Julie A.
(2017)
Apolipoprotein E (epsilon) genotype has a greater impact on apoB-48 than apoB-100 responses to dietary fat manipulation—insights from the SATgenε study.
Molecular Nutrition & Food Research, 61 (4).
ISSN 1613-4125
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Abstract
Scope: To determine the contribution of intestinally and liver-derived lipoproteins to the postprandial plasma triacylglycerol (TAG) response in APOE3/E3 and E3/E4 individuals following chronic dietary fat manipulation. Methods and results: In sequential order, participants (n = 12 E3/E3, n = 11 E3/E4) followed low fat (LF); high-fat, high-saturated fat (HSF); and HSF with 3.45 g/day docosahexaenoic acid (HSF-DHA) diets, each for 8 weeks. After each dietary period, an acute test meal with a macronutrient profile representative of the dietary intervention was consumed. Apolipoprotein (apo)B isoforms were determined in isolated TAG-rich lipoprotein fractions (Sf>400, Sf 60–400 and Sf 20–60) by specific ELISA. A genotype*meal/diet interaction for the Sf>400 fraction apoB-48 response (P<0.05) was observed, with higher concentrations reached after the LF than HSF-DHA meal in E4 carriers. This finding was associated with a lower TAG content of the Sf>400 particles. Fasting Sf 60–400 and 20–60 apoB-48 concentrations were also significantly higher in E4 carriers. No impact of genotype on the apoB-100 responses was evident. Conclusion: Our study revealed marked effects of dietary fat composition on the Sf>400 apoB-48 response and particle TAG content in E4 carriers relative to the ‘wild-type’ E3/E3 genotype, which suggest APOE genotype is a potential modulator of chylomicron particle synthesis.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | apoe genotype,dietary fat quantity,docosahexaenoic acid,saturated fat,triacylglycerol |
| Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Groups > Nutrition and Preventive Medicine Faculty of Medicine and Health Sciences > Research Groups > Cardiovascular and Metabolic Health Faculty of Medicine and Health Sciences > Research Centres > Lifespan Health |
| Related URLs: | |
| Depositing User: | Pure Connector |
| Date Deposited: | 14 Dec 2016 00:07 |
| Last Modified: | 19 Oct 2023 01:53 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/61717 |
| DOI: | 10.1002/mnfr.201600688 |
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