PerR controls oxidative stress defence and aerotolerance but not motility-associated phenotypes of Campylobacter jejuni

Handley, Rebecca A, Mulholland, Francis, Reuter, Mark, Ramachandran, Vinoy K, Musk, Heather, Clissold, Leah, Le Brun, Nick E and van Vliet, Arnoud H M (2015) PerR controls oxidative stress defence and aerotolerance but not motility-associated phenotypes of Campylobacter jejuni. Microbiology, 161. pp. 1524-1536. ISSN 1350-0872

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    Abstract

    The foodborne bacterial pathogen Campylobacter jejuni is an obligate microaerophile, which is exposed to atmospheric oxygen during transmission through the food chain. Survival under aerobic conditions requires the concerted control of oxidative stress systems, which in C. jejuni are intimately connected with iron metabolism via the PerR and Fur regulatory proteins. Here we have characterised the roles of C. jejuni PerR in oxidative stress- and motility phenotypes, and its regulon at the level of transcription, protein expression and promoter interactions. Insertional inactivation of perR in the C. jejuni reference strains NCTC 11168, 81-176 and 81116 did not result in any growth deficiencies, but strongly increased survival in atmospheric oxygen conditions, and allowed growth around filter discs infused with up to 30% H2O2 (8.8 M). Expression of catalase, alkyl hydroperoxide reductase, thioredoxin reductase and the Rrc desulforubrerythrin were increased in the perR mutant, and this was mediated at the transcriptional level as shown by electrophoretic mobility shift assays of the katA, ahpC and trxB promoters using purified PerR. Differential RNA-seq analysis of a fur perR mutant allowed the identification of eight previously unknown transcription start sites of genes controlled by either Fur and/or PerR. Finally, inactivation of perR in C. jejuni did not result in reduced motility, and did not reduce killing of Galleria melonella wax moth larvae. In conclusion, PerR plays an important role in controlling oxidative stress resistance and aerobic survival of C. jejuni, but this role does not extend into control of motility and associated phenotypes.

    Item Type: Article
    Faculty \ School: Faculty of Science > School of Chemistry
    University of East Anglia > Faculty of Science > Research Groups > Biophysical Chemistry
    Faculty of Science
    Faculty of Science > School of Biological Sciences
    Faculty of Medicine and Health Sciences > Norwich Medical School
    ?? RGCOASC ??
    Depositing User: Pure Connector
    Date Deposited: 01 Dec 2015 07:22
    Last Modified: 25 Jul 2018 10:48
    URI: https://ueaeprints.uea.ac.uk/id/eprint/55519
    DOI: 10.1099/mic.0.000109

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