Base-modified NAD and AMP derivatives and their activity against bacterial DNA ligases

Pergolizzi, Giulia, Cominetti, Marco, Butt, Julea N., Field, Robert A., Bowater, Richard and Wagner, Gerd K. (2015) Base-modified NAD and AMP derivatives and their activity against bacterial DNA ligases. Organic & Biomolecular Chemistry, 13. pp. 6380-6398. ISSN 1477-0520

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Abstract

We report the chemical synthesis and conformational analysis of a collection of 2-, 6- and 8-substituted derivatives of β-NAD+ and AMP, and their biochemical evaluation against NAD+-dependent DNA ligases from Escherichia coli and Mycobacterium tuberculosis. Bacterial DNA ligases are validated anti-microbial targets, and new strategies for their inhibition are therefore of considerable scientific and practical interest. Our study includes several pairs of β-NAD+ and AMP derivatives with the same substitution pattern at the adenine base. This has enabled the first direct comparison of co-substrate and inhibitor behaviour against bacterial DNA ligases. Our results suggest that an additional substituent in position 6 or 8 of the adenine base in β-NAD+ is detrimental for activity as either co-substrate or inhibitor. In contrast, substituents in position 2 are not only tolerated, but appear to give rise to a new mode of inhibition, which targets the conformational changes these DNA ligases undergo during catalysis. Using a molecular modelling approach, we highlight that these findings have important implications for our understanding of ligase mechanism and inhibition, and may provide a promising starting point for the rational design of a new class of inhibitors against NAD+-dependent DNA ligases.

Item Type: Article
Uncontrolled Keywords: dna ligase,bioorganic chemistry,nucleic acids,nad,biochemistry,organic chemistry ,/dk/atira/pure/subjectarea/asjc/1300/1303
Faculty \ School: Faculty of Science > School of Biological Sciences
Faculty of Science > School of Pharmacy
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Faculty of Science > School of Chemistry
Depositing User: Pure Connector
Date Deposited: 24 Jul 2015 23:32
Last Modified: 11 Apr 2019 14:52
URI: https://ueaeprints.uea.ac.uk/id/eprint/53591
DOI: 10.1039/c5ob00294j

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