Purinergic-induced signaling in C11-MDCK cells inhibits the secretory Na-K-Cl cotransporter

Brindikova, Tatyana A, Bourcier, Nathalie, Torres, Brian, Pchejetski, Dmitry, Gekle, Michel, Maximov, Georgy V, Montminy, Valérie, Insel, Paul A, Orlov, Sergei N and Isenring, Paul (2003) Purinergic-induced signaling in C11-MDCK cells inhibits the secretory Na-K-Cl cotransporter. American Journal of Physiology - Cell Physiology, 285 (6). C1445-53. ISSN 0363-6143

Full text not available from this repository. (Request a copy)

Abstract

Purinergic inhibition of Na-K-Cl cotransport has been noted in various renal epithelial cells derived from the collecting tubule, including Madin-Darby canine kidney (MDCK) cells. In recent studies, we have observed purinergic inhibition of Na-K-Cl cotransport in C11-MDCK subclones (alpha-intercalated-like cells). Interestingly, Na-K-Cl cotransport activity was also detected in C7-MDCK subclones (principal-like cells) but was not affected by ATP. In this investigation, we have transfected the human Na-K-Cl cotransporter (huNKCC1) in both C11 and C7 cells to determine whether these differences in NKCC regulation by ATP were due to cell-specific purinoceptor signaling pathways or to cell-specific isoforms/splice variants of the transporter. In both cell lines, we found that endogenous as well as huNKCC1-derived cotransport activity was restricted to the basolateral side. In addition, we were able to show that extracellular application of 100 microM ATP or 100 microM UTP abolished NKCC activity in both mock- and huNKCC1-transfected C11 cells but not in mock- and huNKCC1-transfected C7 cells; in C11 cells, intriguingly, this inhibition was not affected by inhibitors of RNA and protein synthesis and occurred even though expression levels of UTP-sensitive P2Y2-, P2Y4-, and P2Y6-purinoceptors were not different from those observed in C7 cells. These results suggest that C11 cells express an undetermined type of UTP-sensitive P2-purinoceptors or a unique P2Y-purinoceptor-triggered signaling cascade that leads to inhibition of NKCC1.

Item Type: Article
Uncontrolled Keywords: adenosine triphosphate,animals,cell line,dogs,epithelial cells,humans,kidney tubules, collecting,membrane potentials,polymerase chain reaction,purines,receptors, purinergic,signal transduction,sodium-potassium-chloride symporters,transfection
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
Depositing User: Pure Connector
Date Deposited: 16 Dec 2014 10:06
Last Modified: 25 Jul 2018 10:20
URI: https://ueaeprints.uea.ac.uk/id/eprint/51551
DOI: 10.1152/ajpcell.00386.2002

Actions (login required)

View Item