Release of angiogenic growth factors from cells encapsulated in alginate beads with bioactive glass

Keshaw, Hussila, Forbes, Alastair and Day, Richard M (2005) Release of angiogenic growth factors from cells encapsulated in alginate beads with bioactive glass. Biomaterials, 26 (19). pp. 4171-9. ISSN 0142-9612

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Abstract

Attempts to stimulate therapeutic angiogenesis using gene therapy or delivery of recombinant growth factors, such as vascular endothelial growth factor (VEGF), have failed to demonstrate unequivocal efficacy in human trials. Bioactive glass stimulates fibroblasts to secrete significantly increased amounts of angiogenic growth factors and therefore has a number of potential applications in therapeutic angiogenesis. The aim of this study was to assess whether it is possible to encapsulate specific quantities of bioactive glass and fibroblasts into alginate beads, which will secrete growth factors capable of stimulating angiogenesis. Human fibroblasts (CCD-18Co) were encapsulated in alginate beads with specific quantities of 45S5 bioactive glass and incubated in culture medium (0-17 days). The conditioned medium was collected and assayed for VEGF or used to assess its ability to stimulate angiogenesis by measuring the proliferation of human dermal microvascular endothelial cells. At 17 days the beads were lysed and the amount of VEGF retained by the beads measured. Fibroblasts encapsulated in alginate beads containing 0.01% and 0.1% (w/v) 45S5 bioactive glass particles secreted increased quantities of VEGF compared with cells encapsulated with 0% or 1% (w/v) 45S5 bioactive glass particles. Lysed alginate beads containing 0.01% and 0.1% (w/v) 45S5 bioactive glass contained significantly more VEGF (p

Item Type: Article
Uncontrolled Keywords: alginates,bioreactors,cell culture techniques,cell line,cell proliferation,cell survival,ceramics,drug delivery systems,drug implants,endothelial cells,fibroblasts,glass,glucuronic acid,hexuronic acids,humans,microspheres,neovascularization, physiologic,tissue engineering,vascular endothelial growth factor a
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
Depositing User: Pure Connector
Date Deposited: 06 Aug 2014 11:48
Last Modified: 11 Apr 2019 15:41
URI: https://ueaeprints.uea.ac.uk/id/eprint/49632
DOI: 10.1016/j.biomaterials.2004.10.021

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