A simple enzyme-linked immunosorbent assay for sex hormone-binding globulin

Iqbal, M J, Forbes, A, Valyani, S H, Corbishley, T P and Wilkinson, M L (1988) A simple enzyme-linked immunosorbent assay for sex hormone-binding globulin. Steroids, 51 (5-6). pp. 599-607. ISSN 0039-128X

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Abstract

A simple enzyme-linked immunosorbent assay (ELISA) for sex hormone-binding globulin (SHBG) has been developed. Polyclonal antibody raised to SHBG purified to homogeneity was employed. The ELISA, which may be performed in under 4 h, shows no cross-reactivity with other serum proteins, has a sensitivity of less than 1.2 fmol per sample, demonstrates excellent correlation with ligand-binding techniques (r = 0.996; p less than 0.0001), and has intra- and inter-assay coefficients of variation of between 5-9% and 7-11% respectively.

Item Type: Article
Uncontrolled Keywords: animals,enzyme-linked immunosorbent assay,ligands,rabbits,reproducibility of results,sex hormone-binding globulin
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
University of East Anglia > Faculty of Medicine and Health Sciences > Research Groups > Gastroenterology and Gut Biology
University of East Anglia > Faculty of Medicine and Health Sciences > Research Groups > Nutrition
Depositing User: Pure Connector
Date Deposited: 06 Aug 2014 11:48
Last Modified: 25 Jul 2018 09:52
URI: https://ueaeprints.uea.ac.uk/id/eprint/49513
DOI:

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