A simple enzyme-linked immunosorbent assay for sex hormone-binding globulin

Iqbal, M. Jawed, Forbes, Alastair ORCID: https://orcid.org/0000-0001-7416-9843, Valyani, Shahin H., Corbishley, Timothy P. and Wilkinson, Mark L. (1988) A simple enzyme-linked immunosorbent assay for sex hormone-binding globulin. Steroids, 51 (5-6). pp. 599-607. ISSN 0039-128X

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Abstract

A simple enzyme-linked immunosorbent assay (ELISA) for sex hormone-binding globulin (SHBG) has been developed. Polyclonal antibody raised to SHBG purified to homogeneity was employed. The ELISA, which may be performed in under 4 h, shows no cross-reactivity with other serum proteins, has a sensitivity of less than 1.2 fmol per sample, demonstrates excellent correlation with ligand-binding techniques (r = 0.996; p less than 0.0001), and has intra- and inter-assay coefficients of variation of between 5-9% and 7-11% respectively.

Item Type:	Article
Uncontrolled Keywords:	animals,enzyme-linked immunosorbent assay,ligands,rabbits,reproducibility of results,sex hormone-binding globulin
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Groups > Nutrition and Preventive Medicine Faculty of Medicine and Health Sciences > Research Groups > Gastroenterology and Gut Biology
Depositing User:	Pure Connector
Date Deposited:	06 Aug 2014 10:48
Last Modified:	24 Oct 2022 23:59
URI:	https://ueaeprints.uea.ac.uk/id/eprint/49513
DOI:	10.1016/0039-128X(88)90055-4

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