3D structure of a heparin mimetic analogue of a FGF-1 activator. A NMR and molecular modelling study

Muñoz-García, Juan C, Solera, Cristina, Carrero, Paula, de Paz, José L, Angulo, Jesús and Nieto, Pedro M (2013) 3D structure of a heparin mimetic analogue of a FGF-1 activator. A NMR and molecular modelling study. Organic & Biomolecular Chemistry, 11 (47). pp. 8269-8275. ISSN 1477-0520

Full text not available from this repository. (Request a copy)

Abstract

The motional behaviour of heparin oligosaccharides in solution is best described as a top rotor having two perpendicular rotation axes. This prevents an accurate extraction of interprotonic distances by NOESY/ROESY based methods. In this paper, we describe the solution structure of the hexasaccharide 1 calculated from high exactitude distance data obtained from off-resonance ROESY combined with a long MD simulation of 500 ns. In previous studies, we have found that two synthetic hexasaccharides having the sulphate groups directed towards one side of its central plane have an opposite biological activity, while 1 is unable to activate the FGF-1 signalling pathway, the other (2) is even more active than the regular region derived hexasaccharide (3) that mimics the natural active compound, heparin. From the structural analysis it was concluded that 1 has similar three-dimensional characteristics to 2 or 3 and therefore the differences in the activity should be due to the arrangement of the sulphate groups within the hexasaccharidic sequence.

Item Type: Article
Uncontrolled Keywords: chemistry,carbohydrate conformation,metabolism,chemistry,models, molecular,molecular dynamics simulation,molecular sequence data,nuclear magnetic resonance, biomolecular,chemistry,drug effects
Faculty \ School: Faculty of Science > School of Pharmacy
Related URLs:
Depositing User: Pure Connector
Date Deposited: 12 May 2014 17:06
Last Modified: 11 Apr 2019 15:39
URI: https://ueaeprints.uea.ac.uk/id/eprint/48068
DOI: 10.1039/c3ob41789a

Actions (login required)

View Item