Control of Turnip yellows virus: Assessing impact on oilseed rape quality traits and dissecting circulative transmission by aphids

Coleman, Alexander (2013) Control of Turnip yellows virus: Assessing impact on oilseed rape quality traits and dissecting circulative transmission by aphids. Doctoral thesis, University of East Anglia.

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    Abstract

    Abstract
    Turnip yellows virus (TuYV) is one of the most significant viral diseases of
    oilseed rape and may be one of main reasons why commercial oilseed rape crops do not
    reach their genetic potential. TuYV is transmitted by aphids, sap-sucking hemipteroid
    insects, and the green peach aphid (GPA) is the predominant vector. TuYV can reduce
    oilseed rape yield by up to 26% in the UK and may also affect oil quality. Current
    control measures rely on insecticides; however, changing legislation and reduced
    effectiveness necessitate novel approaches to virus control. In this thesis, the impact of
    TuYV on the UK commercial oilseed rape crop was established and sources of partial
    resistance to TuYV and aphids were investigated. TuYV reduces yield and has a subtle
    impact on seed physiology including small changes to fatty acid profiles and
    glucosinolate content. Furthermore, these changes appear to be genotype-dependent and
    not as a result of virus accumulation in the plant. To learn more about TuYV
    transmission by aphids, a novel, functional-genomics tool was developed to silence
    aphid genes by plant-mediated RNA interference (PMRi). Highly specific protein
    interactions between virus particles and aphid proteins are critical determinants of
    circulative transmission, a process whereby virus particles can move between aphid cell
    layers. However, the aphid components underlying these processes are poorly
    understood. As the GPA Rack1 protein has been implicated in transcytosis of TuYV
    particles across the aphid gut barrier, PMRi was used to dissect its role in the circulative
    transmission process. This revealed that Rack1 may have a direct role in TuYV
    acquisition by GPA. This work further demonstrates the potential of PMRi as a postgenomics
    tool in aphids and similar insects, but also as a direct means of aphid and/or
    virus control. These contrasting research strategies have provided a two-pronged
    approach towards improving TuYV control.

    Item Type: Thesis (Doctoral)
    Faculty \ School: Faculty of Science > School of Biological Sciences
    Depositing User: Mia Reeves
    Date Deposited: 06 Mar 2014 13:13
    Last Modified: 06 Mar 2014 13:13
    URI: https://ueaeprints.uea.ac.uk/id/eprint/47976
    DOI:

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