A cholesterol-free, high-fat diet suppresses gene expression of cholesterol transporters in murine small intestine

de Vogel-van den Bosch, Heleen M, de Wit, Nicole J W, Hooiveld, Guido J E J, Vermeulen, Hanneke, van der Veen, Jelske N, Houten, Sander M, Kuipers, Folkert, Müller, Michael and van der Meer, Roelof (2008) A cholesterol-free, high-fat diet suppresses gene expression of cholesterol transporters in murine small intestine. AJP: Gastrointestinal and Liver Physiology, 294 (5). G1171-1180. ISSN 0193-1857

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Abstract

Transporters present in the epithelium of the small intestine determine the efficiency by which dietary and biliary cholesterol are taken up into the body and thus control whole-body cholesterol balance. Niemann-Pick C1 Like Protein 1 (Npc1l1) transports cholesterol into the enterocyte, whereas ATP-binding cassette transporters Abca1 and Abcg5/Abcg8 are presumed to be involved in cholesterol efflux from the enterocyte toward plasma HDL and back into the intestinal lumen, respectively. Abca1, Abcg5, and Abcg8 are well-established liver X receptor (LXR) target genes. We examined the effects of a high-fat diet on expression and function of cholesterol transporters in the small intestine in mice. Npc1l1, Abca1, Abcg5, and Abcg8 were all downregulated after 2, 4, and 8 wk on a cholesterol-free, high-fat diet. The high-fat diet did not affect biliary cholesterol secretion but diminished fractional cholesterol absorption from 61 to 42% (P

Item Type: Article
Uncontrolled Keywords: atp binding cassette transporter 1,atp-binding cassette transporters,animals,bile acids and salts,cholesterol,dna-binding proteins,dietary fats,epithelial cells,feces,gene expression regulation,hydroxycholesterols,hydroxymethylglutaryl coa reductases,intestinal absorption,intestine, small,lipoproteins,male,membrane transport proteins,mice,mice, inbred c57bl,mice, knockout,models, biological,oligonucleotide array sequence analysis,orphan nuclear receptors,receptors, cytoplasmic and nuclear,reverse transcriptase polymerase chain reaction,triglycerides
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
Depositing User: Pure Connector
Date Deposited: 21 Feb 2014 11:18
Last Modified: 18 Sep 2018 15:32
URI: https://ueaeprints.uea.ac.uk/id/eprint/47714
DOI: 10.1152/ajpgi.00360.2007

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