Oit1/Fam3D, a gut-secreted protein displaying nutritional status-dependent regulation

de Wit, Nicole J W, IJssennagger, Noortje, Oosterink, Els, Keshtkar, Shohreh, Hooiveld, Guido J E J, Mensink, Ronald P, Hammer, Sebastiaan, Smit, Johannes W A, Müller, Michael and van der Meer, Roelof (2012) Oit1/Fam3D, a gut-secreted protein displaying nutritional status-dependent regulation. Journal of Nutritional Biochemistry, 23 (11). pp. 1425-1433. ISSN 0955-2863

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Abstract

Oncoprotein-induced transcript 1 (Oit1) was previously identified as a dietary fat-induced gene in the small intestine of C57Bl/6J mice. In this study, we further characterized Oit1 and its human ortholog family with sequence similarity 3, member D (Fam3D), on the messenger RNA as well as the protein level. Oit1 and Fam3D were found to be predominantly expressed in the gastrointestinal tract of mice and humans, respectively. Dietary fat induced a clear and acute up-regulation of Oit1, especially in the jejunum, whereas fasting led to a reduced gene expression in the small intestine. Regarding protein expression, we found a remarkable pattern of Oit1 along the longitudinal axis of the intestine, a predominant villus-restricted expression in the proximal small intestine and a more pronounced crypt expression in the distal parts of the intestine. Using transfection experiments, we confirmed secretion of the Oit1 protein, as was predicted by a signal peptide sequence. Detection of Oit1 and Fam3D in plasma samples indicated that both proteins are secreted to the basolateral site of enterocytes. Moreover, in human plasma samples, we also found an effect of nutritional status on Fam3D levels, with a postprandial elevation and a reduction after fasting. In conclusion, Oit1 and Fam3D are gut-derived proteins that are expressed and secreted in a nutritional status-dependent manner.

Item Type: Article
Additional Information: Copyright © 2012 Elsevier Inc. All rights reserved.
Uncontrolled Keywords: adolescent,adult,aged,animals,colon,cytokines,diet, high-fat,fasting,female,gene expression regulation,humans,intestine, small,male,mice,mice, inbred c57bl,mice, knockout,middle aged,nutritional status,ppar alpha,young adult
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
Depositing User: Pure Connector
Date Deposited: 10 Jun 2014 21:32
Last Modified: 21 Mar 2019 11:09
URI: https://ueaeprints.uea.ac.uk/id/eprint/47661
DOI: 10.1016/j.jnutbio.2011.09.003

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