Stable isotope probing - linking microbial identity to function

Dumont, Marc G and Murrell, J Colin (2005) Stable isotope probing - linking microbial identity to function. Nature Reviews Microbiology, 3 (6). pp. 499-504. ISSN 1740-1526

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Abstract

Stable isotope probing (SIP) is a technique that is used to identify the microorganisms in environmental samples that use a particular growth substrate. The method relies on the incorporation of a substrate that is highly enriched in a stable isotope, such as (13)C, and the identification of active microorganisms by the selective recovery and analysis of isotope-enriched cellular components. DNA and rRNA are the most informative taxonomic biomarkers and (13)C-labelled molecules can be purified from unlabelled nucleic acid by density-gradient centrifugation. The future holds great promise for SIP, particularly when combined with other emerging technologies such as microarrays and metagenomics.

Item Type:	Article
Uncontrolled Keywords:	bacteria,carbon isotopes,dna, bacterial,isotope labeling,phylogeny,polymerase chain reaction,rna, ribosomal, 16s
Faculty \ School:	Faculty of Science > School of Environmental Sciences
UEA Research Groups:	Faculty of Science > Research Centres > Centre for Ecology, Evolution and Conservation Faculty of Science > Research Groups > Environmental Biology Faculty of Science > Research Groups > Resources, Sustainability and Governance (former - to 2018) Faculty of Science > Research Groups > Marine and Atmospheric Sciences (former - to 2017)
Depositing User:	Pure Connector
Date Deposited:	10 Feb 2014 15:24
Last Modified:	24 Oct 2022 06:04
URI:	https://ueaeprints.uea.ac.uk/id/eprint/47523
DOI:	10.1038/nrmicro1162

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