Tools
ToolsJüllig, Mia, Hickey, Anthony J, Middleditch, Martin J, Crossman, David J, Lee, Stanley C and Cooper, Garth J S (2007) Characterization of proteomic changes in cardiac mitochondria in streptozotocin-diabetic rats using iTRAQ™ isobaric tags. Proteomics - Clinical Applications, 1 (6). pp. 565-76. ISSN 1862-8346
Full text not available from this repository.Abstract
Diabetes now affects more than 5% of the world's population and heart failure is the most common cause of death amongst diabetic patients. Accumulating evidence supports a view that myocardial mitochondrial structural and functional changes are central to the onset of diabetic heart failure, but the exact nature of these changes at the proteomic level remains unclear.Here we report on proteomic changes in diabetic rat heart mitochondria following 120 days of streptozotocin-diabetes using the recently developed iTRAQ™ labeling method, which permits quantification of proteins directly from complex mixtures, bypassing the limitations associated with gel-based methods such as 2-DE. Of 252 unique proteins identified, 144 were represented in at least three of six individual paired experiments. Relative amounts of 65 proteins differed significantly between the groups, confirming that the cardiac mitochondrial proteome is indeed impacted by diabetes. The most significant changes were increased protein levels of enzymes involved in mitochondrial oxidation of long-chain fatty acids, which was also confirmed by enzyme assays, and decreased levels of multiple enzymes involved in oxidative phosphorylation and catabolism of short-chain fatty acids and branched-chain amino acids. We also found significant changes in levels of several enzymes linked to oxidative stress.
| Item Type: | Article |
|---|---|
| Additional Information: | Copyright © 2007 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
| Uncontrolled Keywords: | sdg 3 - good health and well-being ,/dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being |
| Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Groups > Norwich Clinical Trials Unit |
| Depositing User: | Pure Connector |
| Date Deposited: | 20 Jan 2014 16:04 |
| Last Modified: | 24 Oct 2022 05:34 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/46201 |
| DOI: | 10.1002/prca.200600831 |
Actions (login required)
 |
View Item |