Analysis of epithelial cell shedding and gaps in the intestinal epithelium

Duckworth, Carrie A and Watson, Alastair J (2011) Analysis of epithelial cell shedding and gaps in the intestinal epithelium. Methods in Molecular Biology, 763. pp. 105-114. ISSN 1940-6029

[img] Microsoft Word (Duckworth and Watson 2009) - Draft Version
Download (2621kB)

    Abstract

    The intestinal barrier is formed by a monolayer of columnar epithelial cells. This barrier is effectively maintained despite the high turnover of epithelial cells in the gut. Defects in the mechanism by which barrier function is maintained are believed to play a central role in the pathogenesis of inflammatory bowel disease (IBD). Proinflammatory cytokines such as TNF-α and IFN-γ are often elevated in inflamed tissue of patients with IBD. In fact, anti-TNF-α therapy is routinely administered to patients with Crohn's disease. We have previously demonstrated that intestinal epithelial cells are shed from the intestine leaving a 'gap' in the epithelium that is able to maintain barrier function. The rate of cell shedding and barrier permeability is substantially increased by the administration of TNF-α. Loss of barrier function at the site of a gap may provide a site of entry for disease-causing bacteria.

    Item Type: Article
    Uncontrolled Keywords: acriflavine,animals,crohn disease,cyclic amp,dextrans,female,fluorescein,fluorescent dyes,goblet cells,humans,interferon-gamma,intestine, small,isoquinolines,male,mice,mice, inbred c57bl,microscopy, confocal,permeability,tumor necrosis factor-alpha
    Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
    Depositing User: Users 2731 not found.
    Date Deposited: 14 Nov 2011 13:07
    Last Modified: 09 Apr 2019 10:51
    URI: https://ueaeprints.uea.ac.uk/id/eprint/35440
    DOI: 10.1007/978-1-61779-191-8_7

    Actions (login required)

    View Item