Identification of novel gammadelta T-cell subsets following bacterial infection in the absence of Vgamma1+ T cells:homeostatic control of gammadelta T-cell responses to pathogen infection by Vgamma1+ T cells

Newton, Darren J, Andrew, Elizabeth M, Dalton, Jane E, Mears, Rainy and Carding, Simon R (2006) Identification of novel gammadelta T-cell subsets following bacterial infection in the absence of Vgamma1+ T cells:homeostatic control of gammadelta T-cell responses to pathogen infection by Vgamma1+ T cells. Infection and Immunity, 74 (2). pp. 1097-1105. ISSN 0019-9567

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Abstract

Although gammadelta T cells are a common feature of many pathogen-induced immune responses, the factors that influence, promote, or regulate the response of individual gammadelta T-cell subsets to infection is unknown. Here we show that in the absence of Vgamma1+ T cells, novel subsets of gammadelta T cells, expressing T-cell receptor (TCR)-Vgamma chains that normally define TCRgammadelta+ dendritic epidermal T cells (DETCs) (Vgamma5+), intestinal intraepithelial lymphocytes (iIELs) (Vgamma7+), and lymphocytes associated with the vaginal epithelia (Vgamma6+), are recruited to the spleen in response to bacterial infection in TCR-Vgamma1-/- mice. By comparison of phenotype and structure of TCR-Vgamma chains and/or -Vdelta chains expressed by these novel subsets with those of their epithelium-associated counterparts, the Vgamma6+ T cells elicited in infected Vgamma1-/- mice were shown to be identical to those found in the reproductive tract, from where they are presumably recruited in the absence of Vgamma1+ T cells. By contrast, Vgamma5+ and Vgamma7+ T cells found in infected Vgamma1-/- mice were distinct from Vgamma5+ DETCs and Vgamma7+ iIELs. Functional analyses of the novel gammadelta T-cell subsets identified for infected Vgamma1-/- mice showed that whereas the Vgamma5+ and Vgamma7+ subsets may compensate for the absence of Vgamma1+ T cells by producing similar cytokines, they do not possess cytocidal activity and they cannot replace the macrophage homeostasis function of Vgamma1+ T cells. Collectively, these findings identify novel subsets of gammadelta T cells, the recruitment and activity of which is under the control of Vgamma1+ T cells.

Item Type: Article
Uncontrolled Keywords: amino acid sequence,animals,base sequence,female,gene rearrangement, gamma-chain t-cell antigen receptor,genes, t-cell receptor gamma,homeostasis,humans,listeria monocytogenes,listeriosis,lymphocyte activation,macrophages, peritoneal,male,mice,mice, inbred c57bl,molecular sequence data,receptors, antigen, t-cell, gamma-delta,sequence analysis, dna,spleen,t-lymphocyte subsets
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
University of East Anglia > Faculty of Medicine and Health Sciences > Research Groups > Gastroenterology and Gut Biology
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Depositing User: Rhiannon Harvey
Date Deposited: 14 Jul 2011 12:31
Last Modified: 25 Jul 2018 06:02
URI: https://ueaeprints.uea.ac.uk/id/eprint/33727
DOI: 10.1128/IAI.74.2.1097-1105.2006

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