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Dhanji, Hiran, Murphy, Niamh M., Akhigbe, Christine, Doumith, Michel, Hope, Russell, Livermore, David M. 
ORCID: https://orcid.org/0000-0002-9856-3703 and Woodford, Neil
(2011)
Isolation of fluoroquinolone-resistant O25b:H4-ST131 Escherichia coli with CTX-M-14 extended-spectrum β-lactamase from UK river water.
Journal of Antimicrobial Chemotherapy, 66 (3).
pp. 512-516.
ISSN 0305-7453
Abstract
Objectives We analysed water sampled from the River Thames in London for Escherichia coli resistant to oxyimino-cephalosporins and/or fluoroquinolones, particularly seeking isolates with CTX-M extended-spectrum ß-lactamases (ESBLs) and members of the clinically important O25b:H4-ST131 lineage. Methods River water was collected from three urban sites on the River Thames by the City of London Port Health Authority on two occasions 1 week apart. Coliforms and E. coli were identified by the Quanti-Tray™ method. Disc susceptibility tests were performed and MICs were determined for E. coli isolates resistant to either ciprofloxacin or cefpodoxime and genetic relatedness was determined by PFGE and real-time PCR. PCR was used for phylogenetic and plasmid typing, to detect antibiotic resistance genes and to detect ISEcp1 upstream of blaCTX-M genes. blaCTX-M alleles were identified by sequencing. Results The mean E. coli count, as the most probable number, from the first river samples, taken on a falling tide on 23 March 2010, was 4.7?×?104/100 mL and 30 ciprofloxacin-resistant colonies were isolated. Twenty of the 30 colonies belonged to clone ST131; 10 of these had blaCTX-M-14 whereas the remaining 10 lacked ESBLs. The ST131 isolates represented two different PFGE types. No ciprofloxacin- or cefpodoxime-resistant E. coli were isolated from the second river sample taken at low tide. CTX-M-15, the most common ESBL in clinical E. coli, was not detected in the river samples. Conclusions Water from the River Thames in West London is contaminated, perhaps transiently, with antibiotic-resistant E. coli belonging to the clinically important O25b:H4-ST131 lineage.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | anti-bacterial agents,bacterial typing techniques,drug resistance, bacterial,electrophoresis, gel, pulsed-field,escherichia coli,fluoroquinolones,humans,london,microbial sensitivity tests,molecular typing,plasmids,polymerase chain reaction,rivers,beta-lactamases,beta-lactams |
| Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Groups > Public Health and Health Services Research (former - to 2023) Faculty of Medicine and Health Sciences > Research Groups > Epidemiology and Public Health |
| Depositing User: | Rhiannon Harvey |
| Date Deposited: | 13 Jul 2011 11:04 |
| Last Modified: | 11 Jan 2024 01:20 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/33601 |
| DOI: | 10.1093/jac/dkq472 |
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