Improved growth of enteric adenovirus type 40 in a modified cell line that can no longer respond to interferon stimulation

Sherwood, Victoria, Burgert, Hans-Gerhard, Chen, Yun-Hsiang, Sanghera, Sandeep, Katafigiotis, Socrates, Randall, Richard E., Connerton, Ian and Mellits, Kenneth H. (2007) Improved growth of enteric adenovirus type 40 in a modified cell line that can no longer respond to interferon stimulation. Journal of General Virology, 88 (1). pp. 71-76. ISSN 0022-1317

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Abstract

Human enteric adenoviruses propagate poorly in conventional human cell lines used to grow other adenovirus serotypes. As human enteric adenoviruses have a defect in counteracting the cellular interferon (IFN) response in cell culture, to aid in growth of the virus, a 293-based cell line defective in its ability to respond to IFN was constructed. This cell line (293-SV5/V) constitutively expresses V-protein of the paramyxovirus Simian virus 5, which degrades the signal transducer and activator of transcription 1 (STAT1) and thereby prevents the STAT1-mediated IFN response. Analysis of human enteric adenovirus type 40 (HAdV-40)-infected 293-SV5/V cells compared with parental 293 cells shows that the recombinant line allows more rapid production of virus and results in higher titres. These results suggest that the defect in HAdV-40 in counteracting the IFN response can be overcome at least partially through the use of 293-SV5/V cell lines.

Item Type: Article
Uncontrolled Keywords: activation,gastroenteritis,sensitivity,virus-replication,contribute,promoter,degradation,proteins,simian-virus-5,stat1
Faculty \ School: Faculty of Science > School of Pharmacy
Depositing User: Rachel Smith
Date Deposited: 07 Jun 2011 17:02
Last Modified: 10 Apr 2019 12:30
URI: https://ueaeprints.uea.ac.uk/id/eprint/31977
DOI: 10.1099/vir.0.82445-0

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