Reactions of Nitric Oxide and Oxygen with the Regulator of Fumarate and Nitrate Reduction, a Global Transcriptional Regulator, during Anaerobic Growth of Escherichia coli

Crack, Jason, Le Brun, Nick E., Thomson, Andrew J., Green J, J and Jervis, A (2008) Reactions of Nitric Oxide and Oxygen with the Regulator of Fumarate and Nitrate Reduction, a Global Transcriptional Regulator, during Anaerobic Growth of Escherichia coli. Methods in Enzymology, 437. pp. 191-209. ISSN 1557-7988

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Abstract

The Escherichia coli fumarate and nitrate reductase (FNR) regulator protein is an important transcriptional regulator that controls the expression of a large regulon of more than 100 genes in response to changes in oxygen availability. FNR is active when it acquires a [4Fe-4S]2+ cluster under anaerobic conditions. The presence of the [4Fe-4S]2+ cluster promotes protein dimerization and site-specific DNA binding, facilitating activation or repression of target promoters. Oxygen is sensed by the controlled disassembly of the [4Fe-4S]2+ cluster, ultimately resulting in inactive, monomeric, apo-FNR. The FNR [4Fe-4S]2+ cluster is also sensitive to nitric oxide, such that under anaerobic conditions the protein is inactivated by nitrosylation of the iron-sulfur cluster, yielding a mixture of monomeric and dimeric dinitrosyl-iron cysteine species. This chapter describes some of the methods used to produce active [4Fe-4S] FNR protein and investigates the reaction of the [4Fe-4S]2+ cluster with nitric oxide and oxygen in vitro.

Item Type: Article
Faculty \ School: Faculty of Science > School of Chemistry
University of East Anglia > Faculty of Science > Research Groups > Biophysical Chemistry
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Depositing User: Rachel Smith
Date Deposited: 07 Apr 2011 15:11
Last Modified: 25 Jul 2018 05:16
URI: https://ueaeprints.uea.ac.uk/id/eprint/28490
DOI: 10.1016/S0076-6879(07)37011-0

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