Escherichia coli cytochrome c nitrite reductase NrfA

Clarke, TA, Mills, PC, Poock, SR, Butt, JN, Cheesman, MR, Cole, JA, Hinton, JC, Hemmings, AM, Kemp, G, Soderberg, CA, Spiro, S, Van Wonderen, J and Richardson, DJ (2008) Escherichia coli cytochrome c nitrite reductase NrfA. Methods in Enzymology, 437. pp. 63-77. ISSN 1557-7988

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The periplasmic cytochrome c nitrite reductase (Nrf) system of Escherichia coli utilizes nitrite as a respiratory electron acceptor by reducing it to ammonium. Nitric oxide (NO) is a proposed intermediate in this six-electron reduction and NrfA can use exogenous NO as a substrate. This chapter describes the method used to assay Nrf-catalyzed NO reduction in whole cells of E. coli and the procedures for preparing highly purified NrfA suitable for use in kinetic, spectroscopic, voltammetric, and crystallization studies.

Item Type: Article
Faculty \ School: Faculty of Science > School of Biological Sciences
Faculty of Science > School of Chemistry
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Depositing User: EPrints Services
Date Deposited: 01 Oct 2010 14:36
Last Modified: 11 Apr 2019 14:40
DOI: 10.1016/S0076-6879(07)37004-3

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