DNA adenine methylation regulates virulence gene expression in Salmonella enterica serovar Typhimurium

Balbontin, R., Rowley, G., Pucciarelli, M.G., Lopez-Garrido, J., Wormstone, Y., Lucchini, S., Garcia-Del Portillo, F., Hinton, J.C. and Casadesus, J. (2006) DNA adenine methylation regulates virulence gene expression in Salmonella enterica serovar Typhimurium. Journal of Bacteriology, 188 (23). pp. 8160-8168. ISSN 0021-9193

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Abstract

Transcriptomic analyses during growth in Luria-Bertani medium were performed in strain SL1344 of Salmonella enterica serovar Typhimurium and in two isogenic derivatives lacking Dam methylase. More genes were repressed than were activated by Dam methylation (139 versus 37). Key genes that were differentially regulated by Dam methylation were verified independently. The largest classes of Dam-repressed genes included genes belonging to the SOS regulon, as previously described in Escherichia coli, and genes of the SOS-inducible Salmonella prophages ST64B, Gifsy-1, and Fels-2. Dam-dependent virulence-related genes were also identified. Invasion genes in pathogenicity island SPI-1 were activated by Dam methylation, while the fimbrial operon std was repressed by Dam methylation. Certain flagellar genes were repressed by Dam methylation, and Dam- mutants of S. enterica showed reduced motility. Altered expression patterns in the absence of Dam methylation were also found for the chemotaxis genes cheR (repressed by Dam) and STM3216 (activated by Dam) and for the Braun lipoprotein gene, lppB (activated by Dam). The requirement for DNA adenine methylation in the regulation of specific virulence genes suggests that certain defects of Salmonella Damâ?» mutants in the mouse model may be caused by altered patterns of gene expression.

Item Type: Article
Faculty \ School: Faculty of Science > School of Biological Sciences
University of East Anglia > Faculty of Science > Research Groups > Molecular Microbiology
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Depositing User: EPrints Services
Date Deposited: 01 Oct 2010 14:38
Last Modified: 25 Jul 2018 06:03
URI: https://ueaeprints.uea.ac.uk/id/eprint/1472
DOI: 10.1128/JB.00847-06

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